三叶木通果皮中绿原酸提取工艺的正交试验优选  

Optimization of the extraction technology of chlorogenic acid from Akebia trifoliate peels by orthogonal test

作　　者：金洪光[1] 李玉全[1] 李同建[1] 徐玲玲[1] 江慎华[1] 廖亮[1] 

机构地区：[1]九江学院药学与生命科学学院,江西九江332000

出　　处：《时珍国医国药》2017年第6期1306-1307,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.31660104;No.31460073);江西省自然科学基金(No.20161BAB204165);江西省科技计划项目(No.20142BBF60055);江西省教育厅科学技术研究项目(No.GJJ151090);江西省卫生计生委项目(No.2014A146;No.2014A085)

摘　　要：目的优选三叶木通果皮中绿原酸的最佳提取工艺。方法采用单因素试验和正交试验,考察乙醇浓度、固液比、提取时间和提取温度对绿原酸提取率的影响。结果最佳提取工艺为提取温度90℃,固液比1∶20,乙醇浓度为65%,提取时间2h。结论该工艺操作简单、合理,可为大生产提供参考。Objective To optimally select the best extracting technology of chlorogenic acid in Akebia trifoliate peels by orthogonal test. Methods Single factor and orthogonal experiment method was used for studying the effect of alcohol concentration, the ratio of solid to liquid, extracting time and extraction temperature. Results The optimum extraction technology was as follows： extrac- tion temperature at 90℃, ratio of solid to liquid at 1： 20, concentration of ethanol at 65%, extraction time at 2h. Conclusion The extraction technology is appropriate and feasible, method can provide a scientific basis for manufacture.

关 键 词：三叶木通 绿原酸 正交试验 

分 类 号：R283[医药卫生—中药学]