绵羊虱蝇中肠和蛹菌群结构的分析  

Identification of the microflora in the midgut and pupa of Melophagus ovinus

作　　者：汪雅琴[1,2] 程天印[1,2] 段德勇[1,3,2] WANG Ya-qing CHENG Tian-yin DUAN De-yong(College of Veterinary Medicine ,Hunan Agricultural University, Changsha 410128, China College of Oriental Science ＆ Technology ,Hunan Agricultural University, Changsha 410128, China Hunan Co-Innovation Center of A nimal Production Safety, Changsha 410128, China)

机构地区：[1]湖南农业大学动物医学院,湖南长沙410128 [2]湖南畜禽安全生产协同创新中心,湖南长沙410128 [3]湖南农业大学东方科技学院,湖南长沙410128

出　　处：《中国兽医科学》2017年第7期861-865,共5页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31372431);湖南农业大学东方科技学院青年科学基金项目(16QNZ13)

摘　　要：为探明绵羊虱蝇雌、雄成虫中肠及蛹的菌群结构特点,提供防控绵羊虱蝇及其传播疾病的科学依据,本研究在无菌条件下采集雌、雄绵羊虱蝇成虫中肠内容物及蛹内容物,用试剂盒提取细菌总DNA,运用通用引物PCR扩增细菌16S r DNA V3区,DGGE电泳,选取优势条带,回收、测序,测序结果与Gen Bank数据库中已知序列进行比对。结果显示,雌、雄绵羊虱蝇成虫中肠及蛹的优势菌均为巴尔通氏体、杀雄菌、沃尔巴克氏体。研究结果表明,雌、雄绵羊虱蝇成虫中肠及蛹的菌群结构相似,且巴尔通氏体、杀雄菌、沃尔巴克氏体可垂直传播。The characteristics of microflora in the midgut and pupa of Melophagus ovinus were analyzed by using denaturing gradient gel electrophoresis（DGGE）.The samples of the midgut and pupa contents were collected under aseptic conditions,total DNA of bacteria was extracted according to the manufacturer＇s protocol,and the universal primers were used to amplify the bacteria of V3 areas of 16 S rDNA.The predominance bands were cut for recovery and sequencing after DGGE,and sequencing results were compared with the known sequences in Gen Bank.In result,the dominant bacteria in the midgut and pupa of M.ovinus were Bartonella,Arsenophonus and Wolbachia. The results indicated that microflora in the midgut of female and male adults of M.ovinus were similar to the pupa,Bartonella,Arsenophonus and Wolbachia,and had capability to vertical transmission.

关 键 词：DGGE 绵羊虱蝇 中肠 蛹 菌群结构 

分 类 号：S855.91[农业科学—临床兽医学]