乙肝病毒大蛋白检测及其与HBV-DNA复制的相关性  

The correlation between HBV-DNA replication and HBV-large envelope protein detection

作　　者：韩富秋[1] 董海荣[1] 嘎勒登玛[1] 

机构地区：[1]呼和浩特市第一医院,内蒙古呼和浩特010030

出　　处：《包头医学院学报》2017年第7期34-36,共3页Journal of Baotou Medical College

摘　　要：目的:探讨慢性乙肝感染患者血清乙肝病毒大蛋白(HBV large envelope protein,HBV-LP)对于判断乙肝病毒复制的临床意义。方法:选择540例慢性乙肝(chronic hepatitis B,CHB)患者和100例健康体检人群,通过Elisa方法检测HBV-LP和HBe Ag的含量,采用实时荧光定量PCR方法检测HBV-DNA的含量。结果:HBe Ag阳性标本中,HBVDNA和HBV-LP的阳性率分别为97.6%和91.6%,差异有统计学意义(P<0.05);HBe Ag阴性标本中,HBV-DNA和HBV-LP的阳性率分别为67.7%和70.8%,差异无统计学意义(P>0.05);所有患者中,HBV-DNA和HBV-LP的阳性率分别为81.5%和80.4%,差异无统计学意义(P>0.05)。DNA拷贝数与HBV-LP的阳性率呈正相关(r=0.865,P<0.05);DNA的拷贝数与HBV-LP的吸光度(A值)呈正相关(r=0.897,P<0.05)。结论:慢性乙肝患者,血清HBV-LP的含量与HBV-DNA的含量密切相关,可以作为判断HBV-DNA复制的指标之一。Objective ： To explore the clinical significance of HBV large envelope protein （ HBV - LP） in the diagnosis of HBV replication in chronic hepatitis B （CHB） patients. Methods： The levels of HBV - LP and HBeAg were detected by enzyme - linked immunosorbent assay （ ELISA ） in 540 cases of CHB patients and 100 cases of healthy individuals. Serum HBV - DNA level was quantitatively detected using real - time polymerase chain reaction （PCR）. Results： In HBeAg positive samples, the positive rates of HBV - DNA and HBV -LP were 97.6 % and 91. 6 %, which had significant difference （ P 〈 0.05 ）. In HBeAg negative samples, the positive rates of HBV - DNA and HBV - LP were 67.7 % and 70.8 % , which had no significant difference （ P 〉0.05）. In all patients, the positive rates of HBV - DNA and HBV - LP were 81.5 % and 80.4 % , which had no significant difference （ P 〉 0.05 ）. The positive rate of HBV - LP level was closely correlated with the number of HBV - DNA copies （ r = 0. 865, P 〈 0.05） and the HBV - DNA copies was closely correlated with HBV - LP A values（ r = 0. 897, P 〈 0.05 ）. Con- clusion ： There is a close correlation between the copies of HBV - DNA and the levels of HBV - LP, which may be used as one of markers to detect HBV replication.

关 键 词：乙型肝炎病毒 乙肝病毒大蛋白 乙肝病毒DNA 

分 类 号：R512.62[医药卫生—内科学]