低温胁迫对番茄RNA解旋酶SlDEAD34基因表达的影响  

Gene Expression Analysis of RNA Helicase Gene SlDEAD34 under Low Temperature Stress in Tomato

作　　者：许瑞瑞[1] 李高青[2] 刘彩云[1] 阚世红[1] 刘春香[1] XU Rui-rui LI Gao-qing LIU Cai yun KAN Shi-hong LIU Chun-Jiang(Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong, College of Biological and Agricultural Engineering,Weifang University,Weifang 261061, China Agricultural Bureau of Shouguang, Weifang 262700, China)

机构地区：[1]潍坊学院生物与农业工程学院山东省高校生物化学与分子生物学重点实验室,山东潍坊261061 [2]寿光市农业局,山东潍坊262700

出　　处：《上海交通大学学报（农业科学版）》2017年第3期76-82,共7页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家自然科学基金青年项目(31400225);山东省优秀中青年科学家科研奖励基金项目(BS2014SW014)

摘　　要：利用生物信息学方法和实时荧光定量PCR技术对番茄RNA解旋酶SlDEAD34的蛋白结构特征、SlDEAD34基因的组织表达模式和低温胁迫条件下的基因表达情况进行了研究。结果表明,SlDEAD34包含9个保守基序,是DEAD-box型RNA解旋酶,与拟南芥LOS4高度同源,三维结构非常类似;SlDEAD34基因在生长6周的番茄根、茎和叶中均有表达,且根>茎>叶,在生长5个月的番茄9种组织器官中均有表达,其中在果实中表达量最高,在根中表达量最低;4℃处理时,番茄根中SlDEAD34基因下调明显,叶中表达量变化不明显,12℃处理时,番茄根和叶中SlDEAD34基因表达量变化均不明显。DEAD-box型RNA解旋酶SlDEAD34经低温4℃处理后基因表达量明显下调,暗示SlDEAD34基因响应低温胁迫,推测SlDEAD34基因可能参与调控低温逆境胁迫过程。Bioinformatics and quantitative RTPCR were employed to study the protein structure of S1DEAD34 and the expression pattern of SlDEAD34 in different vegetative organs under low temperature stress in tomato. Results showed that S1DEAD34,highly homologous with Arabidopsis LOS4, is a DEAD box RNA helicase with 9 conserved motifs. SlDEAD34 expresses in all tested tissues of 6 weeks seedling, with the highest level in root and lowest in leaf,whereas with the highest level in fruit and lowest in root of 5 months mature plants. Interestingly, the expression levels of SlDEAD34 in root were much lower than in leaf under the low temperature stress of 4℃ However, the gene expression of SlDEAD34 has no obvious change in both root and leaf under 12 ℃ treatment in tomato. In conclusion, SlDEAD34 was downregulated significantly after low temperature 4 ℃ treatment. Taken together, we speculated that SlDEAD34 might be involved in the response of tomato to low temperature stress.

关 键 词：番茄 RNA解旋酶 SlDEAD34基因 低温胁迫 基因表达 

分 类 号：S641.2[农业科学—蔬菜学]