右旋糖酐-40葡萄糖注射液中葡萄糖的含量测定  

Determination of Glucose in Dextran-40 Glucose Injection by HPLC

作　　者：李娜 

机构地区：[1]焦作市食品药品检验所,河南焦作454001

出　　处：《抗感染药学》2017年第3期491-494,共4页Anti-infection Pharmacy

摘　　要：目的:建立右旋糖酐-40葡萄糖注射液中葡萄糖含量的高效液相色谱测定法。方法:采用高效液相色谱法,色谱柱采用磺化交联苯乙烯-二乙烯共聚合物的氢型阳离子交换树脂为填充剂(300 mm×7.8 mm);流速为0.6 m L/min;柱温为40℃;采用示差折光检测器检测,进样量为20μL。结果:葡萄糖浓度在1~10 mg/m L范围内呈良好的线性关系,葡萄糖的平均回收率为102.23%(RSD=0.70%,n=9),测得右旋糖酐-40葡萄糖注射液中葡萄糖含量平均为51.33 mg/m L。结论:本方法灵敏度高、操作简单、准确可靠,适用于高效液相色谱法测定右旋糖酐-40葡萄糖注射液中葡萄糖的含量。Objective： To establish a method for determination of glucose in dextran-40 glucose in- jection by HPLC. Methods：High performance liquid chromatography column for the sulfonated/diphe- nyl ethylene-divinyl hydrogen type cation exchange resin was as filling agent of polymer （300 mm× 7.8 mm）. Flow rate was 0.6 mL/min. Column temperature was 40℃. In determination by refractive index detector, sample quantity was 20 μL. Results： Glucose levels were between 1 and 10 mg/mL and had a good linear relationship. Glucose of the average recovery was 102.23% （RSD=0.70%, n =9）. Glucose contents were measured with an average to 51.33 mg/mL. Conclusion： This method is of high sensitivity, operated simply, accurate and reliable. And it is suitable for glucose content are determination in dextran 40 injection by HPLC. KEY WORDS Dextran 40 glucose; content determination; glucose; high performance liquid chromatogmphy （HPLC）

关 键 词：右旋糖酐40葡萄糖注射液 含量测定 葡萄糖 高效液相色谱法 

分 类 号：R969.3[医药卫生—药理学]