明胶纳米颗粒的制备  

Preparation of Gelatin Nanoparticles

作　　者：杨岚[1] 王春华[1] 李昌朋[2] 林炜[1] YANG Lan WANG Chun-hua LI Chang-peng LIN Wei(Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, Sichuan University, Chengdu 610065, Sichuan, China China Academy of Engineering Physics Research Center of Laser Fusion, Mianyang, 621000, Sichuan, China)

机构地区：[1]四川大学皮革化学与工程教育部重点实验室,四川成都610065 [2]中国工程物理研究院激光聚变研究中心,四川绵阳621000

出　　处：《精细化工》2017年第7期774-779,共6页Fine Chemicals

基　　金：国家自然科学基金资助项目(21476148);四川大学研究生课程建设项目(2016KCJS121)~~

摘　　要：以B型明胶为原料,戊二醛为交联剂,通过改进的两步去溶剂法制备了较小粒径(约100 nm)的明胶纳米颗粒(Gelatin nanoparticles,GNPs),考察了分散体系浓度、去溶剂化试剂、交联剂用量对GNPs粒径的影响。结果表明:降低明胶在二次分散体系中的浓度,制得的GNPs的尺寸更小且分布均匀。残留的去溶剂化试剂丙酮需去除,因为它会使GNPs分散性变差。在明胶质量浓度为8 g/L的分散液中,当戊二醛加入量为4.5%(以明胶的质量为基准,下同)时,即可制得稳定的GNPs,其粒径为50~150 nm,PDI约为0.14,Zeta电位绝对值大于30 m V。AFM和TEM测试结果表明:GNPs呈光滑球形,为边缘部分比核心更为疏松的非均质结构。Gelatin nanoparticles（GNPs,about 100 nm in diameter） were prepared by using type B gelatin as raw material,glutaraldehyde as crosslinking agent by a modified two-step desolvation process.The effects of the gelatin concentration in dispersion solution,desolvation solvent（acetone）,and the amount of the cross-linker（glutaraldehyde） on the particle size of GNPs were also investigated. The results showed that the GNPs with a smaller particle size and uniform distribution can be obtained by reducing the content of gelatin in the second dispersed solution before crosslinking. Moreover,the residual acetone in the as-prepared dispersion must be removed completely. Otherwise,it can lead to poor distribution of GNPs. Stable GNPs were obtained（diameter of 50 ~ 150 nm,polydispersity index of about 0. 14,and Zeta potential 30mV） when the dosage of glutaraldehyde was 4. 5%（based on mass of gelatin） in 8 g/L gelatin dispersion. The results from atomic force microscope（AFM） and transmission electron microscope（TEM） showed that the as-prepared GNPs exhibited spherical morphology with a smooth surface. Additionally,the edge part of heterogeneous sphere was looser than the core.

关 键 词：明胶纳米颗粒 两步去溶剂法 去离子水 戊二醛 功能材料 

分 类 号：TB34[一般工业技术—材料科学与工程]