HPLC-DAD-ESI-Q-TOF/MS法测定金银忍冬花中的化学成分  

Determination of chemical constituents in Lonicera maackii with HPLC-DAD-ESI-Q-TOF/MS

作　　者：朱姮 崔莉[1] 刘倩[1] 姜姣姣[2] 文蕾[2] 耿岩玲[1] 王晓[1] 赵恒强[1] 

机构地区：[1]山东省中药质量控制技术重点实验室,山东省科学院中药过程控制研究中心山东省分析测试中心,山东济南250014 [2]山东中医药大学药学院,山东济南250355

出　　处：《中草药》2017年第11期2300-2305,共6页Chinese Traditional and Herbal Drugs

基　　金：山东省科技发展计划(2014GSF119031,2016GSF202014);山东省泰山学者岗位资助(郭兰萍);山东省科学院先导专项(郭兰萍);中央本级重大增减支项目“名贵中药资源可持续利用能力建设”(2060302);国家自然基金重点项目“中药道地性及其形成的环境机制研究”(81130070)

摘　　要：目的运用HPLC-DAD-ESI-Q-TOF/MS法鉴别和测定金银忍冬中主要化学成分。方法采用HPLC-DAD-ESI-Q-TOF/MS技术对金银忍冬中化学成分进行鉴别,采用HPLC-DAD法分析测定金银忍冬中的主要化学成分的量。结果从金银忍冬中检测到31个色谱峰,并鉴定出19种化学成分,测定了金银忍冬3个花期中10种主要成分(马钱酸、绿原酸、马钱苷、莫诺苷、断氧化马钱苷、芦丁、金丝桃苷、木犀草苷、异绿原酸A、异绿原酸C)的量。结论金银忍冬花蕾期的主要化学成分量高于白花期和黄花期;金银忍冬花蕾与金银花中10种化学成分的量及分布特征相近;为开发利用金银忍冬药用资源提供数据支持。Objective To identify and determine the main chemical constituents of Lonicera maackii, and to provide data support for the development and utilization of L. maackii. Methods The chemical constituents of L. maackii were identified by HPLC-DAD-ESI-Q-TOF/MS and the main chemical constituents were determined by HPLC-DAD. Results A total of 31 chromatographic peaks were detected and 19 chemical constituents were identified from L. maackii, and the contents of the 10 compounds（logaric acid, chlorogenic acid, loganin, morroniside, Secoxyloganin, rutin, hyperoside, luteoloside, chlorogenic acid A, and chlorogenic acid C） were determined. Conclusion The contents of the main chemical constituents in the flower buds of L. maackii were higher than those in the blooming stage and yellow flowering stage. The contents of main components in L. maackiiwere similar to those of Lonicerae Japonicae Flos. The results of this study provide data support for the development and utilization of L. maackii.

关 键 词：金银忍冬 HPLC-DAD-ESI-Q-TOF/MS 金银花 有机酸类 环烯醚萜苷类 黄酮类 马钱酸 绿原酸 

分 类 号：R284.1[医药卫生—中药学]