藤梨根总黄酮对大鼠肝脏组织抗氧化能力及其相关基因表达的影响  

Effects of total flavonoids of Actinidiae Radix on anti-oxidant capacity and related gene expression in rat liver tissues

作　　者：滕坤[1] 臧皓[1] 沈鹏[1] 张海丰[1] 阮洪生[2] 

机构地区：[1]通化师范学院制药与食品科学学院,吉林通化134002 [2]浙江医药高等专科学校中药学院,浙江宁波315100

出　　处：《中草药》2017年第12期2486-2491,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31100254);吉林省教育厅项目(吉教科合字[2015]第439号)

摘　　要：目的对藤梨根总黄酮(TFAR)体内与体外的抗氧化活性及其相关机制进行研究。方法以清除1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基能力为指标对TFAR进行体外抗氧化活性实验;以D-半乳糖致衰老大鼠为模型,测定大鼠肝脏组织中丙二醛(MDA)的量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活力及抗氧化相关基因SOD1、SOD2、GPX1 mRNA表达。结果 TFAR对DPPH、ABTS自由基具有良好的清除作用,IC_(50)分别为27.01 mg/mL和29.55 mg/mL。与模型组比较,TFAR各给药组肝脏组织中MDA、SOD、CAT指标均有显著改善(P<0.05、0.01),表现在MDA水平降低,SOD、CAT活性升高,并回调到对照组水平以上。TFAR各剂量组对指标的调控作用呈量效关系。与模型组比较,TFAR 3个剂量组均能够上调衰老模型大鼠肝脏组织上清液中SOD1、SOD2、GPX1mRNA的表达,差异显著(P<0.05、0.01),且存在一定的剂量依赖关系。结论 TFAR在体外和体内均有抗氧化活性,抗氧化活性与上调大鼠肝脏组织中SOD1、SOD2、GPX1 mRNA表达有关。Objective To investigate the antioxidant activities of total flavonoids of Actinidiae Radix（TFAR） in vitro and in vivo and the related mechanism. Methods The anti-oxidant activities of TFAR were determined using DPPH and ABTS radical scavenging assays in vitro. The content of MDA and the activity of SOD and CAT in rat liver tissue were determined. Results TFAR have showed significant DPPH radical scavenging activity and ABTS free radical scavenging activity and the IC（50） values of TFAR were 27.01 and 29.55 mg/mL, respectively. Compared with the model group, the MDA content of liver tissue was significantly decreased in the order of TFAR from low dose to high dose, and the activity of SOD and CAT increased significantly. Compared with model group, gene expression of mRNA of SOD1, SOD2 and GPX1 in liver tissue was up-regulated by each dose group of TFAR. Conclusion TFAR has anti-oxidant activity in vitro and in vivo, and has a dose-dependent relationship with the expression of mRNA in rat liver tissue.

关 键 词：藤梨根 总黄酮 抗氧化 丙二醛 超氧化物歧化酶 过氧化氢酶 

分 类 号：R285.5[医药卫生—中药学]