基于生物信息学方法探析川芎嗪对类风湿性关节炎作用机制  

Mechanism of Tetramethylpyrazine on Rheumatoid Arthritis Based on Bioinformatics Method

作　　者：白菁安[1] 边艳琴[2] 牛旭艳[1] 樊丹平 吕诚[1] 姜淼[1] 赵宁[1] BAI Jing＇an BIAN Yanqin NIU Xuyan FAN Danping LYU Cheng JIANG Miao ZHAO Ning(Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China Shuguang Hospital Affiliated of Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)

机构地区：[1]中国中医科学院中医临床基础医学研究所,北京100700 [2]上海中医药大学附属曙光医院,上海201203

出　　处：《辽宁中医药大学学报》2017年第7期73-77,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30825047);中国中医科学院院级自主选题项目(Z0412)

摘　　要：目的:通过生物信息学方法探析川芎嗪对类风湿性关节炎作用机制,为其后续的实验验证及应用拓展提供预测性科学依据。方法:将Pubchem数据库中查找到的川芎嗪人类靶蛋白和类风湿性关节炎Gene数据库中获得的人类靶基因导入IPA分析平台,把两者相关的网络、信号通路进行比较分析,进而得出川芎嗪作用于类风湿性关节炎的可能机制。结果:通过本研究,发现川芎嗪主要通过第二信使信号通路参与类风湿性关节炎发生发展中细胞的生长、增殖和发育、生存与死亡等功能。川芎嗪通过三条路径影响了类风湿性关节炎中细胞的转录功能,分别是Gaq-PI3K-AKT-NF-κB路径、Gas-c AMP-RAF-ERK1/2-CREB路径和Gai-STAT3路径。川芎嗪可能通过RGS蛋白对G蛋白偶联受体信号进行了负调控。结论:通过查找川芎嗪靶蛋白、类风湿性关节炎基因并通过IPA软件进行分析可以得出川芎嗪作用于RA的可能机制。Objective：To explore the mechanism of tetramethylpyrazine on rheumatoid arthritis through bioinformatics methods, and provide scientific basis for its follow-up experimental verification and application development. Methods ： The human target gene of tetramethylpyrazine human target protein and rheumatoid arthritis gene were obtained in pubchem database, and the related network and signal pathway were compared, and the possible mechanism of ligustrazine in rheumatoid arthritis was obtained. Results ： Through this study, it was found that tetramethylpyrazine is mainly involved in the growth, value addition and development, survival and death of cells in the development of rheumatoid arthritis through the second messenger signaling pathway. Tetramethylpyrazine affects the transcription of cells in rheumatoid arthritis through three pathways, namely Gaq-PI3K-AKT-NF-κB pathway, Gas-cAMP-RAF-ERK1/2-CREB pathway and Gai-STAT3 pathway. Tetramethylpyrazine may regulate the signal of g protein coupled receptor by rgs protein. Conclusion ： The possible mechanism of tetramethylpyrazine is analyzed by looking for the expression of tetramethylpyrazine target protein, rheumatoid arthritis gene and through IPA software.

关 键 词：川芎嗪 类风湿性关节炎 IPA Pubchem 

分 类 号：R593.22[医药卫生—内科学]