丝裂霉素C抑制大鼠创伤性骨感染软组织瘢痕形成的效果  

Effect of mitomycin C in reducing hypertrophic scar in rat traumatic osteomyelitis model

作　　者：王鹏[1] 宫照燕 宋春好 刘博[1] 陈聪[1] 李伟[1] 殷德振[1] 信效堂 逄凯[1] 徐鹏[1] 穆卫东[4] Wang Peng Gong Zhaoyan Song Chunhao Liu Bo Chen Cong Li Wei Yin Dezhen Xin Xiaotang Pang Kai Xu Peng Mu Weidong(Department of Spine Surgery, Weihai Municipal Hospital, Weihai 264200, Chin)

机构地区：[1]威海市立医院脊柱外科,264200 [2]威海市立医院临床营养科,264200 [3]威海市文登区人民医院介入科 [4]山东省立医院创伤骨科

出　　处：《中华创伤杂志》2017年第7期651-657,共7页Chinese Journal of Trauma

基　　金：国家自然科学基金（81171708,81401786）;山东省重点研发计划（2016GSF201218）

摘　　要：目的探讨丝裂霉素C在创伤性骨感染模型中抑制软组织瘢痕形成的效果及机制。方法120只Wistar大鼠按随机数字表法分为空白对照组（A组）、创伤性骨感染组（B组）、创伤性骨感染丝裂霉素C处理组（C组），每组40只。B组、C组利用金黄色葡萄球菌在骨折基础上制作创伤性骨感染动物模型。伤后15，30d取材，HE染色观察组织结构变化，免疫组化染色检测转化生长因子-β1（TGF-β1）表达，Masson染色观察胶原沉积程度，Westernblot检测TGF-β1及Ⅰ型胶原的表达。结果HE染色显示，A组肌纤维排列整齐；B组伤后15d肌纤维排列紊乱，至伤后30d肌纤维出现漩涡状瘢痕组织；C组肌纤维紊乱程度较B组减轻。伤后15d时，B、C组TGF-β1阳性表达分数均高于A组（P〈0．05），而C组低于B组（P〈0．05）；30d时，B、C组均高于A组（P〈0．05），而C组低于B组（P〈0．05）。伤后15d时，B、C组胶原容积分数均高于A组（P〈0．05），而C组低于B组（P〈0．05）；30d时，B、C组均高于A组（P〈0．05），而C组低于B组（P〈0．05）。伤后15d时，B、C组TGF-β1、Ⅰ型胶原表达积分吸光度值均高于A组（P〈0．05），而C组低于B组（P〈0．05）；30d时，B、C组均高于A组（P〈0．05），而C组低于B组（P〈0．05）。结论丝裂霉素C可通过下调TGF-β1及I型胶原的表达抑制创伤性骨感染周围软组织瘢痕形成，为临床创伤性骨感染的辅助治疗提供新的思路。Objective To investigate the effect and mechanism of mitomycin C in reducing hypertrophic scar in rat traumatic osteomyelitis model. Methods A total of 120 Wistar rats were divided into control group （ Group A, n = 40 ） , traumatic osteomyelitis group （ Group B, n = 40 ） , traumatic osteomyelitis treated with Mitomycin C group （ Group C, n = 40 ） , according to the random number table. The model of traumatic osteomyelitis was produced by Staphylococcus aureus. Muscle tissues around the focus were harvested at 15 d and 30 d postinjury. HE staining was used to observe the changes of muscle tissue structure. Immunohistochemistry was used to detect expression of transforming growth factor （TGF）-β1. Masson staining was used for collagen deposition evaluation. Western blot was used for detection of levels of TGF-β1 and collagen Ⅰ Results HE staining revealed consistent alignment of fibers within the muscle in Group A. Fibrosis with the muscle was observed in both Group B and C, but the degree of muscle fiber disorder was decreased in Group C compared to Group B. Either 15 d or 30 d after injury, expressions intensity of TGF-β1, collagen fraction volume, and activation levels of TGF-β1 as well as collagen Ⅰ were higher in Group B and C than Group A, and all parameters were decreased in Group C compared to Group B （ all P 〈 0.05）. Conclusion Mitomycin C can reduce hypertrophic scar formation in traumatic osteomyelitis model, and the potential mechanism relates to down- regulated TGF--β1 and collagen Ⅰ .

关 键 词：骨髓炎 创伤性 瘢痕 丝裂霉素类 

分 类 号：R681.2[医药卫生—骨科学]