阿萨伊对虚热和虚寒证大鼠的脂肪代谢、免疫物质和内分泌激素的影响  

Effect of Aaí (Euterpe oleracea) on lipid metabolism,immune substances and endocrine hormone in rats with deficiency-heat and deficiency-cold syndrome

作　　者：王子晨[1] 张建军[1] 朱映黎[1] 瞿研[2] 费文婷 王莎[1] 王景霞[1] 王林元[2] 

机构地区：[1]北京中医药大学中医学院,北京100029 [2]北京中医药大学中药学院,北京100102

出　　处：《中国中药杂志》2017年第13期2552-2557,共6页China Journal of Chinese Materia Medica

基　　金：北京中医药大学自主课题(2015-JYB-XS002;2016-JYB-JSPY-013)

摘　　要：研究阿萨伊对虚热证、虚寒证大鼠的脂肪代谢、免疫物质及内分泌激素水平变化。SD大鼠分为空白组、虚热模型组、虚热模型加黄柏组、虚热模型加阿萨伊高、低剂量组、虚寒模型组、虚寒模型加肉桂组、虚寒模型加阿萨伊高、低剂量组;采用每日肌肉注射地塞米松磷酸钠、氢化可的松琥珀酸钠1次、连续21 d的方法制备大鼠虚热模型、虚寒模型;通过比色法检测脂肪代谢(FFA,LPL,HL)指标变化以及免疫(IgG,IgM,C3,C4)指标变化;通过放免法检测内分泌激素(CORT,E2,T)指标变化。阿萨伊高剂量组能降低虚热证大鼠血清中FFA,LPL,HL值(P<0.01或P<0.001);阿萨伊高剂量组能显著升高虚热证大鼠血清中IgG,IgM,C3值(P<0.05或P<0.001);阿萨伊高剂量组能显著升高虚热证大鼠血清中CORT值(P<0.05),能显著降低E2,E2/T值(P<0.05);阿萨伊高剂量组对虚寒模型脂肪代谢指标作用不明显,但可以升高免疫指标IgG,IgM,C3值(P<0.05或P<0.001),同时对内分泌激素指标CORT值也有升高的作用(P<0.05)。阿萨伊的作用趋向与黄柏一致,表现出对虚热模型大鼠的调整作用;与肉桂相反,对虚寒模型大鼠的指标纠正不明显。本实验从同类比较、异类反证角度验证了理论推导的阿萨伊中药寒凉药性。To study the effects of Aaí（Euterpe oleracea） on lipid metabolism,immune substances and endocrine hormone level in rats with deficiency-heat and deficiency-cold syndrome. SD rats were divided into blank control group,deficiency-heat model group,deficiency-heat Phellodendri Cortex group,deficiency-heat Aaí high dose and low dose groups,deficiency-cold model group,deficiency-cold Cinnamomi Cortex group,deficiency-cold Aaí high dose and low dose groups. The rats received intramuscular injection of dexamethasone sodium phosphate（0. 35 mg） or hydrocortisone sodium succinate（20 mg） for 21 days to set up deficiency-heat models and deficiency-cold models. Then the changes in fatmetabolism levels（ FFA,LPL,HL） and immune indexes（ IgG,IgM,C3 and C4） were detected by colorimeter; and the levels of endocrine hormone indexes（ CORT,E2 and T） were detected by radioimmunoassay. The levels of FFA,LPL and HL in serum were reduced（ P〈0. 01 or P〈0. 001）; levels of IgG,IgM and C3 in serum were increased（ P〈0. 05 or P〈0. 001）; level of CORT in serum was increased（ P〈0. 05） and the level of E2,E2/T in serum were reduced in the Aaí high dose group（ P〈0. 05）. The effect of high dose Aaí on fat metabolism was not obvious in deficiency-cold models,but the levels of IgG,IgM,C3 and CORT in serum were increased（ P〈0. 05 or P〈0. 001）. Aaí was showed the same effect trend with Phellodendri Cortex in adjusting the levels of deficiency-heat rats; but unlike Cinnamomi Cortex,Aaí was showed no obvious effect in adjusting the levels of deficiency-cold rats. In this experiment,homogeneous comparison and heterogeneous disproof were used to verify the cold nature of Aaí.

关 键 词：阿萨伊 虚热证 虚寒证 脂肪代谢 免疫物质 内分泌激素 中药药性 

分 类 号：R285.5[医药卫生—中药学]