芎芷地龙汤不同时间预防给药对偏头痛动物模型PKCγ、PKCε mRNA的影响  

Effects of Xiongzhi Dilong Decoction Preadministration on PKCγ and PKCε mRNA in Migraine Animal at Different Time

作　　者：胡坤[1] 王永丽[1] 赵永烈[1,2] 岳广欣[3] HU Kun WANG Yongli ZHAO Yonglie et al(The Third Hospital,Beijing University of Traditional Chinese Medicine ,Beijing 100029 ,China.)

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]北京中医药大学东方医院,北京100078 [3]中国中医科学院,北京100053

出　　处：《中国中医急症》2017年第7期1134-1136,1144,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81373591)

摘　　要：目的观察芎芷地龙汤不同时间预给药对偏头痛动物模型PKCγ、PKCε mRNA表达的影响。方法将健康雄性SD大鼠36只,随机分为生理盐水组、模型组、舒马普坦组、芎芷地龙汤1 d、3 d、7 d预给药组,每组6只。按照预定给药,造模结束后2 h取材,real-time PCR法测定三叉神经节、三叉神经脊束核丘脑、硬脑膜PKCγ、PKCε mRNA表达情况。结果与生理盐水组比较,模型组PKCγ mRNA在三叉神经节、三叉神经脊束核、硬脑膜表达水平明显升高,在丘脑表达水平明显降低(P<0.01),给药干预后,三叉神经节、三叉神经脊束核、硬脑膜PKCγ mRNA表达水平均降低,其中以预防给药7 d组和舒马普坦组降低明显(P<0.01);丘脑PKCγ mRNA表达水平有所升高,以1 d组、3 d组、舒马普坦组明显((P<0.05或P<0.01)。与生理盐水组比较,模型组PKCε mRNA在硬脑膜、三叉神经节、三叉神经脊束核表达水平明显升高(P<0.01),在丘脑表达水平降低(P<0.01);给药干预后,PKCε mRNA在三叉神经节、三叉神经脊束核表达水平降低,以预防给药7 d组和舒马普坦组降低明显(P<0.01),在硬脑膜表达水平降低,以3 d组和舒马普坦组(均P<0.01)较1 d组和7 d组(均P<0.05)明显;PKCε mRNA在丘脑表达水平升高,各组升高水平均有统计学意义(P<0.01)。结论芎芷地龙汤预防给药可以减少NTG诱发的三叉神经节、三叉神经脊束核PKCγ mRNA、PKCε mRNA表达,而以预防给药7 d效果好于预防给药3 d、1 d。Objective： To study the effect of Xiongzhi Dilong Decoction predministration on PKCγ and PKCε mRNA in migraine animal at different time. Methods： 36 healthy male SD rats were randomly divided into six groups： saline group（n = 6）,migraine model group（n = 6）,sumatriptan group（n = 6）,1 day preadministration group（n = 6）,3 day preadministration group（n = 6）,7 day preadministration group（n = 6）. The drug was administered. Tissues were collected 2 h after successful modeling. The expression of PKCγ and PKCε mRNA in trigeminal ganglion,spinal trigeminal nucleus,thalamus and dura mater were assayed with real-time PCR method. Results： Compared with saline group,the expression of PKCγ mRNA in the model group increased significantly in trigeminal ganglion,spinal trigeminal nucleus and dura mater,and decreased significantly in thalamus（P 0.01）.After drug intervention,the expression of PKCγ mRNA decreased in trigeminal ganglion,spinal trigeminal nucleus and dura mater,and the preadministration of 7 days group and sumatriptan group decreased significantly（P 0.01）. The expression of PKCγ mRNA increased in thalamus,and the preadministration of 1 days group（P 0.01）,3 days group（P 0.05） and sumatriptan group（P 0.01） increased significantly. Compared with saline group,the expression of PKCε mRNA in the model group increased significantly in trigeminal ganglion,spinal trigeminal nucleus and dura mater（P 0.01）,and decreased significantly in thalamus（P 0.01）. After drug intervention,the expression of PKCε mRNA decreased in trigeminal ganglion and spinal trigeminal nucleus,and the preadministration of 7 days group and sumatriptan group decreased significantly（ P 0. 01）. The expression of PKCε mRNA decreased in dura mater,and the preadministration of 3 days group（P 0.01） and sumatriptan group（P 0.01） decreased more significantly than preadministration 1 days group（P 0.05） and 7 days group（P 0.05）.The expression of PKCε mRNA increased in thalamus,a

关 键 词：偏头痛 芎芷地龙汤 预防给药 PKCΓ MRNA PKCΕ MRNA 

分 类 号：R285.5[医药卫生—中药学]