荷丹片对动脉粥样硬化ApoE(-/-)小鼠血清IL-1β和TNF-α表达的影响  

The Effect of Hedan Decoction on the Expression of Interleukin-1β and Tumor Necrosis Factor-α of Atherosclerotic in ApoE(-/-) Mice

作　　者：孙龙飞[1,2] 徐风燕[1] 葛振嵘[1] 沈祥礼 安冬青[2,3] SUN Longfei XU Fengyan GE Zhenrong et al(Coronary Care Unit,Af- filiated Hospital of 71 itional Chinese Medicine ,Xinjiang Medical University ,Xinjiang , Urumqi 830000, China)

机构地区：[1]新疆医科大学附属中医医院,新疆乌鲁木齐830000 [2]新疆医科大学中医学院,新疆乌鲁木齐830000 [3]新疆名医名方与特色方剂学重点实验室,新疆乌鲁木齐830000

出　　处：《中国中医急症》2017年第7期1137-1140,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题(81160428)

摘　　要：目的观察荷丹片对载脂蛋白E基因敲除[ApoE(-/-)]小鼠血清中白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)表达的影响,探讨荷丹片防治动脉粥样硬化(AS)的可能机制。方法 48只8周龄ApoE(-/-)小鼠分为正常对照组(12只,给予普通饲料室温饲养),高脂饲料复合气候箱干预组(36只)。喂养12周后,成功复制ApoE(-/-)小鼠动脉粥样硬化秽浊痰阻证模型后,分为3组:模型组、荷丹片组、阿托伐他汀组。12周后处死各组小鼠,采集标本,HE染色光镜下观察主动脉病理学变化,并测定斑块面积(PA)与管腔面积(LA)之比;酶联免疫双抗夹心(ELISA)法检测血清中IL-1β和TNF-α含量。结果模型组主动脉PA/LA比值大于正常对照组(P<0.01),荷丹片组、阿托伐他汀组PA/LA比值则小于模型组(均P<0.01),荷丹片与阿托伐他汀组比较,差异无统计学意义(P>0.05)。模型组血清中IL-1β和TNF-α表达水平均高于正常对照组(均P<0.01),荷丹片组、阿托伐他汀组血清中IL-1β和TNF-α表达水平均低于模型组(均P<0.01),荷丹片组与阿托伐他汀组比较,差异均无统计学意义(均P>0.05)。结论荷丹片具有防治AS的作用,其机制可能与其降低炎症因子IL-1β和TNF-α的表达,抑制炎症反应有关。Objective： To observe the effect of Hedan Decoction on the expression of Interleukin-1β（IL-1β）and Tumor necrosis factor-α（TNF-α） in serum in apolipoprotein E gene knock-out [ApoE（-/-） mice],and to explore the possible mechanism of Hedan Decoction in the prevention and treatment of atherosclerosis. Methods：48 ApoE（-/-） mice of 8 weeks＇ old were divided into two groups： 12 ApoE（-/-） mice in the normal control group fed with normal diet,and 36 in the intervention group with high fat diet in artificial climate box. 36 ApoE（-/-）mice were fed for 12 weeks to duplicate the model of atherosclerosis huizhuotanzu syndrome. Thereafter,they were randomized into three groups： the model group,HDP group and Atorvastatin group. After 12 weeks,the mice were killed and the samples were collected. The pathological changes of aorta were observed under light microscope with HE staining,and the ratio of plaque area and lumen area were measured. Serum IL-1β and TNF-αlevels were measured by enzyme-linked immunosorbent assay. Results： The ratio of aortic plaque area/lumen area in the model group was higher than that in the normal control group（P 0.01）. Compared with the model group,the ratio of aortic plaque area/lumen area in HDP group and atorvastatin group were decreased（P 0.01）;there was no statistically significant difference between HDP group and atorvastatin group（P 0.05）. Levels of serum IL-1β and TNF-α in the model group were significantly higher than those of the normal control group（P 0.01）. Compared with model group,serum IL-1β and TNF-α levels in HDP group and Atorvastatin group reduced obviously（P 0.01）;there was no statistically significant difference between HDP group and atorvastatin group（P 0.05）. Conclusion： Hedan Decoction can prevent atherosclerosis,whose mechanism may be related to the reduction of inflammatory factor IL-1β and TNF-α,so as to reduce the inflammatory response.

关 键 词：荷丹片 动脉粥样硬化 白细胞介素-1Β 肿瘤坏死因子-Α 

分 类 号：R285.5[医药卫生—中药学]