急性脊髓损伤后最佳干预时间窗的实验研究  

Experimental Study of the Proper Time Window of Intervention after Acute Spinal Cord Injury

作　　者：李晓宁[1] 单筱淳 吴磊[2] 梁雪松[2] 迟蕾[2] 刘双岭[1] 梅继林 李诺[2] LI Xi- aoning SHAN Xiaochun WU Lei et al(The Second Affiliated Hospital of Heilong]iang University of Chinese Medicine ,Heilongjiang,Harbin 150001, China)

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国中医急症》2017年第7期1213-1214,1223,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金(面上)项目(81373715)

摘　　要：目的观察急性脊髓损伤(ASCI)后大鼠神经细胞凋亡出现的最早时间,明确早期干预的时间点。方法将48只雌性Wistar大鼠,随机平分为两组:假手术组(Sham)及急性脊髓损伤组(ASCI),每组各分为4个亚组即1 h、3 h、6 h和8 h。于相应时间点进行造模后取材。HE染色法观察大鼠脊髓组织病理学形态变化;TUNEL染色法检测大鼠神经细胞凋亡情况,筛选出神经细胞凋亡出现最早时间点,确定早期干预的最佳时间点。结果 1)HE染色结果显示,ASCI组大鼠术后1 h和3 h可见灰质小灶性斑片状出血;术后6 h细胞形态明显变化,神经细胞出现凋亡;术后8 h细胞损伤加重,形态进一步变小,仅见残存的神经细胞。2)TUNEL检测显示,ASCI组术后6 h细胞形态改变显著,可见凋散在凋亡神经细胞,与Sham组比较有显著差异(P<0.05);8 h后神经凋亡细胞数量明显增多,与Sham组比较具有显著性差异(P<0.05)。结论急性脊髓损伤后6 h内为本病最佳干预时间窗。Objective： To observe the earliest time of neuronal apoptosis in rats after acute spinal cord injury（ASCI）,and to determine the time of early intervention. Methods： 48 female Wister rats were randomly divided into the sham operation group（Sham） and acute spinal cord injury group（ASCI）,and each group was divided into four subgroups：1 h,3 h,6 h and 8 h. The rats were sacrificed at the corresponding time point after the model was made. HE staining was used to observe the pathological changes of the spinal cord in rats,and TUNEL staining was used to detect the apoptosis of rats to select the earliest time point of apoptosis and to determine the best time of early intervention. Results： 1）HE staining showed that 1 h and 3 h after operation,there was Gray matter focal patchy hemorrhage in ASCI group,and 6 h after operation,the cell morphology changed obviously,and the nerve cells showed apoptosis,and 8h after operation,cell morphological damage grew serious,further smaller,only surviving nerve cells. 2）TUNEL assay showed 6 h after operation,the morphological changes of the cells were obvious,and the apoptosis was found in the apoptotic neurons. There was a significant difference compared with the Sham group（P 0.05）. 8 h after operation,the number of apoptotic cells increased significantly. Compared with group Sham,the difference was significant（P 0.05）. Conclusion： The best intervention time window is within six hours after acute spinal cord injury.

关 键 词：脊髓损伤 细胞凋亡 最佳干预时间窗 

分 类 号：R651.2[医药卫生—外科学]