5-羟色胺转运体基因LPR及5-羟色胺1A受体C(-1019)G基因多态性的联合作用与重性抑郁症的关联研究  

Association between the combination of serotonin transporter gene-linked polymorphic region (5-HTT LPR) and serotonin 1A receptor gene C(-1019) G polymorphism with major depressive disorder

作　　者：关小妮[1] 张克让[2] 崔勇[1] 薛德旺[1] 段宏秋[1] 吕广有 修梅红[1] 

机构地区：[1]北京回龙观医院,北京100096 [2]山西医科大学第一医院,山西太原030001

出　　处：《四川精神卫生》2017年第3期248-252,共5页Sichuan Mental Health

摘　　要：目的探讨5-羟色胺转运体基因LPR(5-HTT LPR)多态性和5-羟色胺1A受体(5-HT_(1A)R)C(-1019)G基因多态性的联合作用与重性抑郁症的关系。方法采用病例对照研究,以2005年10月-2007年5月在山西医科大学第一医院就诊的197例重性抑郁症患者为患者组,选取同期该院体检中心与患者组性别、年龄匹配的健康对照者197例为对照组。应用聚合酶链反应技术(PCR)、SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)及DNA直接测序法对两组的5-HTT LPR及5-HT_(1A)R C(-1019)G基因多态性进行检测。应用UNPHASED软件进行统计分析。结果患者组与对照组的5-HTT LPR和5-HT_(1A)R C(-1019)G多态性位点基因型及等位基因分布频率差异均无统计学意义(P均>0.05)。多态性联合作用分析显示,患者组G-S等位基因组合频率高于对照组,差异有统计学意义(21.4%vs.12.4%,P=0.022)。结论在中国汉族人群中,5-HTT LPR和5-HT_(1A)R C(-1019)G的联合作用与重性抑郁症存在关联,G-S等位基因组合可能会增加重性抑郁症发病的危险性。Objective To investigate the relationship between the combination of 5 - HTT LPR and 5 - HT1AR C（ - 1019） G gene polymorphism with major depressive disorder. Methods The case - control study design was adopted in the study. 197 Chinese Han origin patients with major depressive disorder who came from the First Hospital of Shanxi Medical University from October 2005 to May 2007 were recruited as patient group. At the same time, 197 healthy controls matched age and sex frequencies with patient group from the same hospital were included the control group. Polymerase chain reaction （PCR） , sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） and DNA sequencing analysis were used to detect the genotype of 5 - HTT LPR and 5 - HT1AR C（ -1019）G in two groups. The UNPHASED software was used for statistical analysis. Results There was no significant differences of the distribution of genotype and alleles of 5 - HTT LPR, 5 - HT1AR C（ - 1019） G between two groups （all P 〉 0. 05 ）. The gene combination analysis indicated that the frequency of G - S allele combination in patient group was significantly higher than that in the control group （21.4% vs. 12.4%, P = 0.022） . Conclusion This study suggest that the combination of 5 - HTT LPR and 5 - HT1AR C （ - 1019） G gene polymorphism is closely related with major depressive disorder in Chinese Han origin. The G-S allele combination may increase the risk of morbidity of major depressive disorder.

关 键 词：抑郁症 5-羟色胺转运体 5-羟色胺1A受体 基因多态性 

分 类 号：R749.4[医药卫生—神经病学与精神病学]