基于线粒体COⅠ和ND1基因分析三尾凤蝶遗传多样性  

Genetic diversity of Bhutanitis thaidina Blanchard,based on mitochondrial COⅠ and ND1

作　　者：赵健[1] 胡劭骥[2] 和秋菊[1] 易传辉[3] 和菊[3] 冯志伟[3] 杨建华[3] 陈鹏[3] 

机构地区：[1]西南林业大学、云南省森林灾害预警与控制重点实验室,云南昆明650224 [2]云南大学农学院,云南昆明650223 [3]云南省林业科学院,云南昆明650201

出　　处：《福建农林大学学报（自然科学版）》2017年第4期387-391,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(31260527);云南省中青年学术技术带头人后备人才项目(2013HB093);云南省森林灾害预警与控制重点实验室开放基金项目(ZK150005)

摘　　要：以云南和四川分布的3个种群(云南丽江种群、云南东川种群、四川种群)共36头三尾凤蝶为研究对象,选取线粒体COⅠ和ND1基因作为分子标记,对三尾凤蝶的遗传多样性进行了研究.结果表明,PCR扩增测得COⅠ和ND1基因联合序列长度为1 913 bp,A+T含量为74.3%,表现出明显的A/T碱基偏向性;共检测到4个变异位点,定义了5个单倍型,其中,Ha1出现的频率较高,为共享单倍型.核苷酸和单倍型多样性指数分别为0.000 2和0.218 0;遗传分化系数(Gst)、固定系数(Fst)、基因流(Nm)和遗传距离分别为0.059 98、0.059 95、3.920 00和0.分析显示,三尾凤蝶遗传多样性低,各地理种群基因交流频繁,没有形成明显分化.Thirty-six Bhutanitis thaidina samples were collected from 3 geographical populations （ YNLJ, YNDC, SC） in Yunnan and Sichuan Provinces, and mitochondrial COI and ND1 genes were sequenced and analyzed. The results show-ed that the combined mitochondrial COI and ND1 fragment were 1 913 bp in length. Base composition of A and T was 74.3%, indicating a strong A＋T bias. A total of 4 mutation sites and 5 haplotypes including one shared haploid type （Ha1） were detected. The nucleotide and haplotype diversity were 0.000 2 and 0.218 0. Coefficient of genetic differentiation （Gst） , fixation indices （Fst） , gene flow （Nm） and genetic distance were 0.059 98, 0.059 95, 3.920 00 and 0, respectively. The results indicated low genetic diversity and an extensive gene flow among different B. thaidina populations, and gene differentiations were not found among all populations.

关 键 词：三尾凤蝶 线粒体 COI ND1 遗传多样性 遗传分化 

分 类 号：Q963[生物学—昆虫学]