三明野生蕉和天宝蕉对Foc TR4侵染早期应答的差异  

Comparisons of early responses of 'Tianbaojiao' banana and Sanming wild banana to Foc TR4 infection

作　　者：程春振[1] 马文昇 刘炜婳[1] 张梓浩[1] 齐全[1] 孙雪丽[1] 张永艳[1] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2017年第4期397-401,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(31601713);福建省中青年教师教育科研项目(JAT160166);福建农林大学A类人才科研启动基金(61201400707);福建农林大学博士后启动基金(13230097);福建省省级扶贫重点县科技人员专项计划(K15160001A)

摘　　要：以GFP标记的香蕉枯萎病菌Foc TR4作为病原菌,比较了水培条件下Foc TR4对"天宝蕉"和三明野生蕉的早期侵染情况.研究表明,Foc TR4在"天宝蕉"韧皮部的定殖明显早于三明野生蕉.枯萎病菌感染香蕉后会诱发香蕉细胞内活性氧爆发和激活寄主抗氧化系统.通过研究水培和土培条件下两种香蕉种质接种枯萎病菌后根系超氧化物歧化酶(SOD)变化情况,发现三明野生蕉根系SOD活性高于"天宝蕉",三明野生蕉感染枯萎病后根系SOD活性增强,而"天宝蕉"根系SOD活性下降,说明三明野生蕉的耐枯萎病能力可能与其根系较高和早期上调的SOD活性有关.GFP-labeled Foe TR4 was used to infect Sanming wild banana and ‘ Tianbaojiao5 banana,and its colonization was evaluated by spore and root development under laser scanning confocal microscope and superoxide dismutase （SOD） analysis of root. Colonization was first detected in the root phloem of ‘ Tianbaojiao5 banana. Higher SOD activity was found in Sanming wild banana. And opposite changing patterns of SOD activity were found between 2 banana gernplasms,with up-regulation being in Sanming wild ba-nana and down-regulation in ‘ Tianbaojiao banana5. It could be concluded that the higher and up-regulated SOD activity in the early infection stage is attributed to Fusarium wilt tolerance of Sanming wild banana.

关 键 词：香蕉 枯萎病 绿色荧光蛋白 超氧化物歧化酶 早期应答 

分 类 号：S668.1[农业科学—果树学] S436.68[农业科学—园艺学]