芍药甘草汤对脂多糖诱导大鼠背根神经节神经元损伤的保护机制研究  

The Protective Effect of the Extracts of the Chinese Medicine Shaoyao and Gancao to DRGn Damaged by LPS

作　　者：马千[1] 谢晓燕[2] 叶玲[1] 尉明洋[1] 张娟[1] MA Qian XIE Xiaoyan YE Ling et al(Zhejiang Hospital of Traditional Chinese Medicine, Hangzhou 310006 China Taihe Hospital of Shiyart City, Hubei Provine)

机构地区：[1]浙江中医药大学附属第一医院,杭州310006 [2]湖北省十堰市太和医院

出　　处：《浙江中医药大学学报》2017年第6期513-517,共5页Journal of Zhejiang Chinese Medical University

基　　金：国家自然基金(81202823/H2902);浙江省医药卫生科技计划(2014KYA157);浙江省自然基金(LY16H290003)~~

摘　　要：[目的]通过脂多糖(lipopolysaccharide,LPS)诱导背根神经节神经元(dorsal root ganglion neuron,DRGn)发生损伤,探讨芍药甘草汤对DRGn炎性损伤的保护作用及机制。[方法]实验分为4组:空白对照(Con)组、LPS(LPS)组、芍药甘草汤(SGD)组、芍药甘草汤+尼克酰胺(SGD+NAM)组。相差显微镜下观察各组DRGn形态的改变;CCK法检测各组DRGn的存活率,并且通过Western blot法检测各组神经元沉默信息调节因子2(silencing information regulator 2,Sir2)相关酶1(silent mating type information regulation 2 homolog 1,SIRT1)、mTOR、p-mTOR和Ac-NF-κB的表达。[结果]LPS组较Con组DRGn的形态发生明显改变,存活率明显降低(P<0.01),SIRT1和p-mTOR表达降低(P<0.05),mTOR和Ac-NF-κB表达增高(P<0.05);SGD组较LPS组DRGn的存活率增加(P<0.01),SIRT1和p-mTOR表达增加(P<0.05),mTOR和Ac-NF-κB的表达降低(P<0.05);SGD+NAM组较SGD组DRGn的存活率降低(P<0.05),SIRT1和p-mTOR的表达降低(P<0.05),mTOR和Ac-NF-κB的表达增高(P<0.05)。[结论]LPS可诱导背根神经节神经元发生损伤。芍药甘草汤可能是通过增加SIRT1的表达,促进mTOR向p-mTOR转化,降低Ac-NF-κB的表达,进而抑制细胞内后续炎症因子的释放从而发挥其神经元保护作用。[Objective] By establishing the cell model of dorsal root ganglion neuron（DRGn） impaired by lipopolysaccharide（LPS）,to explore the extracts of Chinese medicine Shaoyao and Gancao on DRGn protective effect and mechanism of inflammatory damage.[Method]Cells were divided randomly into four groups： Control group（Con）,LPS group（LPS）, SGD group（SGD）, SGD＋Nicotinamide（SGD＋NAM）.The morphology of the DRGn was observed by microscope and the survival rate of DRGn was detected by CCK-8 test. The expression levels of silent mating type information regulation 2 homolog 1（SIRT1）, mTOR,p-mTOR and Ac-NF-κB were detected by Western blot.[Results]Compared with the Con group： The morphology in the DRGn of the LPS group significantly changed and the survival rate of it declined with the declining expression levels of SIRT1 and p-mTOR, and the elevating expression levels of mTOR and Ac-NF-κB. In contrast to LPS group： the survival rate of DRGn and the expression of SIRT1 and p-mTOR increased in SGD group, and the expressions of mTOR and Ac-NF-κB were lower. However, the survival rate of DRGn and the expression of SIRT1 and p-mTOR declined in SGD＋NAM group, and the expression of mTOR and Ac-NF-κB increased compared with SGD group. [Conclusion]The extracts of the Chinese medicine Shaoyao and Gancao may perform its protective effect through SIRT1-p-mTOR-Ac-NF-κB pathway to DRGn which was impaired by LPS.

关 键 词：芍药甘草汤 神经性病理性疼痛 炎性损伤 DRGn SIRT1 镇痛 Ac-NF-κB mTOR 

分 类 号：R331[医药卫生—人体生理学]