炎症因子在大叶性肺炎患儿支气管肺泡灌洗液及血清中的表达及临床意义  

Clinical Significance and Expression of Inflammatory Factors in Bronchoalveolar Lavage Fluid and Serum in Children with Lobar Pneumonia

作　　者：刘钢[1] 李然然[1] 李萍萍[1] 姜荣燕[1] 陈述英[1] LIU Gang LI Ran-ran LI Ping-ping JIANG Rong-yan CHEN Shu-ying(Department of Pediatn＇cs, Affiliated Hospital of Taishan Medical University, Tai＇an, Shandong, 271000, China)

机构地区：[1]泰山医学院附属医院儿科,山东泰安271000

出　　处：《现代生物医学进展》2017年第20期3917-3920,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨炎症因子在大叶性肺炎患儿支气管肺泡灌洗液及血清中的表达水平及其临床意义。方法:收集2014年6月至2016年6月我院儿科收治的79例大叶性肺炎患儿为病例组,按照临床肺部感染评分(CPIS)分为重度组(n=46)和轻度组(n=33),于同期随机选取30例健康儿童为对照组,采用双抗体夹心酶联免疫吸附法(ELISA)检测各组支气管肺泡灌洗液及血清炎症因子如白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、白细胞介素-17(IL-17)、肿瘤坏死因子(TNF-α)以及高迁移率族蛋白B1(HMGB1)表达水平。结果:重度组、轻度组血清IL-6、IL-8、IL-17、TNF-α及HMGB1水平高于对照组,重度组血清IL-6、IL-8、IL-17、TNF-α及HMGB1水平高于轻度组,差异有统计学意义(P<0.05)。病例组患儿支气管肺泡灌洗液IL-6、IL-8、IL-17、TNF-α及HMGB1水平高于血清,差异有统计学意义(P<0.05)。重度组、轻度组支气管肺泡灌洗液IL-6、IL-8、IL-17、TNF-α及HMGB1水平高于血清,差异有统计学意义(P<0.05),重度组支气管肺泡灌洗液、血清IL-6、IL-8、IL-17、TNF-α及HMGB1水平分别高于轻度组,差异有统计学意义(P<0.05)。经Spearman积矩相关分析,支气管肺泡灌洗液与血清中IL-6、IL-8、IL-17、TNF-α及HMGB1水平分别呈正相关关系(r=0.711、0.695、0.752、0.793、0.728,P<0.05)。结论:炎症因子在大叶性肺炎患儿支气管肺泡灌洗液及血清中呈高表达,并与大叶性肺炎病情严重程度密切相关,联合检测有助于早期评估病情。Objective：To explore the clinical significance and expression of inflammatory factors in bronchoalveolar lavage fluid and serum in children with lobar pneumonia.Methods：79 children with lobar pneumonia were selected as case group,who were divided into severe group （n=46） and mild group （n=33） according to the clinical pulmonary infection score （CPIS）.30 healthy children were randomly selected as control group in the same period.The levels of Inflammatory cytokines such as interleukin-6 （IL-6）,interleukin-8 （IL-8）,interleukin-17（-17）,tumor necrosis factor（TNF-α） and high mobility group box-1（HMGB1） in bronchoalveolar lavage fluid and serum of each group were detected by the enzyme-linked-immunosorbent serologic assay （ELISA）.Results：Serum IL-6,IL-8,IL-17,TNF-α and HMGB1 levels of the severe group and mild group were higher than those of the control group,and the serum IL-6,IL-8,IL-17,TNF-α and HMGB1 levels of the severe group were higher than those of the mild group,the differences were statistically significant（P＜0.05）.The IL-6,IL-8,IL-17,TNF-α and HMGB1 levels of the case group in bronchoalveolar lavage fluid were higher than in the serum,the differences were statistically significant （P＜0.05）.The IL-6,IL-8,IL-17,TNF-α and HMGB1 levels of severe group in bronchoalveolar lavage fluid,mild group were higher than those in serum,the differences were statistically significant（P＜0.05）.The IL-6,IL-8,IL-17,TNF-α and HMGB 1 levels in bronchoalveolar lavage fluid and serum of severe group were higher than those of mild group,the differences were statistically significant （P＜0.05）.Spearman product moment correlation analysis showed that the IL-6,1L-8,IL-17,TNF-oα and HMGB1 levels in bronchoalveolar lavage fluid were positively related with those in serum （r=0.711,0.695,0.752,0.793,0.728,P＜0.05）.Conclusion：Inflammatory factors in bronchoalveolar lavage fluid and serum in children with lobar pneumonia show high expression,which is closely relate

关 键 词：大叶性肺炎 患儿 支气管肺泡灌洗液 血清 炎症因子 

分 类 号：R563.1[医药卫生—呼吸系统]