急性高原性脑水肿小鼠大脑超微结构及咬合蛋白(Occludin)的改变  

Changes of ultrastructure and Occludin in mouse brain of acute high altitude cerebral edema

作　　者：常朋飞[1,2] 刘冀琴[3] 赵娟[2] 韩威[4] 张璐[5] 

机构地区：[1]潍坊市中医院,山东潍坊261041 [2]武警后勤学院临床医学系生理学与病理生理学教研室,天津300309 [3]武警后勤学院附属医院检验科,天津300162 [4]武警后勤学院训练部,天津300309 [5]武警后勤学院科研部,天津300309

出　　处：《武警后勤学院学报（医学版）》2017年第5期397-401,共5页Journal of Logistics University of PAP（Medical Sciences）

基　　金：武警后勤学院博士启动金项目(WHB2013-02;WHB2013-09);武警后勤学院附属医院种子基金项目(FYM201208)

摘　　要：【目的】探讨急性高原性脑水肿小鼠大脑超微结构及咬合蛋白(Occludin)的改变。【方法】成年健康雄性昆明种小鼠随机分为:空白对照组、12 h急性缺氧组和24 h急性缺氧组,适应性饲养1周后放入低压氧舱,建立脑水肿模型。干湿重法测定脑水含量,伊文思蓝法测定血脑屏障通透性,观察脑组织形态和超微结构改变,免疫组化和Western blotting检测Occludin蛋白变化,QPCR检测Occludin m RNA变化。【结果】急性缺氧12 h与24 h组小鼠脑水含量以及EB含量明显升高(P<0.05);光镜下可见缺氧后小鼠海马区神经细胞被破坏,核固缩,部分核仁显示不清,细胞间隙明显增宽,间质疏松,细胞周围及间质水肿;电镜下可见内皮细胞肿胀严重,线粒体肿胀明显,内皮细胞核固缩,紧密连接开放,胶质细胞足突重度水肿,内皮细胞吞饮小泡增多,内质网脱颗粒;急性缺氧后Occludin m RNA及蛋白表达与空白对照组比较均降低(P<0.05),且24 h急性缺氧组低于12 h急性缺氧组(P<0.05)。【结论】急性低压低氧环境可诱发小鼠脑水肿,并可使小鼠脑内神经细胞损伤,开放BBB间的紧密连接。【Objective】To explore the changes of ultrastructure and Occludin in mouse brain of acute high altitude cerebral edema.【Methods】Healthy adult male kunming mice were randomly divided into blank control group, 12 h and 24 h acute hypoxia groups.After 1 w of adaptive feeding, the mice were put into the low-pressure oxygen chamber to make cerebral edema models. Brain water content was measured by dry and wet weight method. Evans blue method was used to determine the permeability of blood brain barrier.The morphological and ultrastructural changes of brain tissues were observed. Immunohistochemistry and Western blotting were used to test the protein change of occludin, and QPCR was used to detect the m RNA change of Occludin.【Results】The contents of water and EB in mouse brain increased significantly in 12 h and 24 h acute hypoxia groups（P〈0.05）. The results in hypoxia groups under light microscope indicated that： hippocampus nerve cells were destroyed; karyopyknosis could be seen; partial nucleoli were not clear;intercellular space widened obviously; interstitial substance loosened; peripheral and interstitial edema could be seen. The results under electron microscope showed that： endothelial cells and mitochondria swelled seriously, karyopyknosis could be seen in endothelial cells;gap junctions opened; severe edema appeared in the podocytic process of glial cells; pinocytosis vesicles in endothelial cells increased;endoplasmic reticulum was degranulated. Compared with the blank control group, the m RNA and protein expressions of Occludin decreased in acute hypoxia groups（P〈0.05）. Moreover, those in 24 h group were lower than those in 12 h group（P〈0.05）.【Conclusion】Acute low-pressure oxygen environment can induce brain edema in mice, make the nerve cells damaged and open gap junctions in BBB.

关 键 词：急性高原性脑水肿 小鼠 大脑 OCCLUDIN 

分 类 号：R34[医药卫生—基础医学]