补气活血通络法对神经吻合术后大鼠神经及腓肠肌蛋白表达的影响  

Study the Effect of Buqi Huoxue Tongluo on the Expression of Protein in the Gastrocnemius Muscle after Sciatic Nerve Anastomosis in Rats

作　　者：刘俊宁[1] 王庆莲[2] 张燕[1] 牛素生[1] 刘宇[1] 

机构地区：[1]福建中医药大学骨伤学院,福建福州350122 [2]福建中医药大学附属第三人民医院脾胃科

出　　处：《潍坊医学院学报》2017年第4期251-254,共4页Acta Academiae Medicinae Weifang

基　　金：福建省自然科学基金资助课题(课题编号:2016J01772);福建中医药大学校管-重点学科专项基金资助课题(课题编号:X2014085)

摘　　要：目的研究补气活血通络法对大鼠坐骨神经吻合术后神经功能及腓肠肌内蛋白表达的影响。方法采用SPF级SD大鼠,雌雄各半,随机分为假手术组、生理盐水组。甲钴胺片组以及补阳还五汤组4组。分别在造模给药后2,4个疗程(每个疗程15d)进行大体观察、电生理观察以及采用Western Bloting法检测FoxO3a和MAFbx的蛋白表达。结果给药4个疗程时补阳还五汤组大鼠肢体功能改善明显好于生理盐水组和甲钴胺片组;神经电生理检测坐骨神经传导速度,神经吻合术后各组均明显差于假手术组,4个疗程时补阳还五汤组和甲钴胺片组神经传导速度均优于相应组别的2个疗程时传导速度;腓肠肌细胞内FoxO3a和MAFbx蛋白的表达在相同的疗程补阳还五汤组和甲钴胺片组明显低于生理盐水组,补阳还五汤组FoxO3a和MAFbx蛋白表达低于甲钴胺片组。结论采用补气活血通络法可明显促进大鼠神经吻合术后功能障碍的恢复,促进神经的修复以及抑制FoxO3a和MAFbx蛋白的表达。Objective To study the effect of Buqi Huoxue Tongluo on the expression of protein in the gastrocnemius muscle after sciatic nerve anastomosis in rats. Methods SPF grade SD rats were randomly divided into sham operation group,normal saline group,mecobalamin group and Buyang Huanwu Decoction group. The expression of FoxO3 a and MAFbx was detected by Western blotting. The expression of FoxO3 a and MAFbx was detected by immunohistochemistry. Results In the course of 4 courses of treatment,the body function of Buyang Huanwu Decoction group was improved obviously,and the dysfunction was significantly better than that of the saline group and the mecobalamin group; Electrosurgical detection of sciatic nerve conduction velocity,the nerve anastomosis were significantly worse than the sham operation group,nerve conduction velocity of 4 courses were superior to the corresponding group of 2 courses of conduction speed; The expression of FoxO3 a and MAFbx protein in the same course of treatment was significantly lower than that in the saline group. Expression of FoxO3 a and MAFbx Proteins in Buyang Huanwu Decoction group was lower than that of mecobalamin tablets. Conclusion The method of Invigorating Qi,activating blood circulation and dredging collaterals can obviously promote the recovery of dysfunction after nerve anastomosis,promote the repair of nerve and inhibit the expression of FoxO3 a and MAFbx protein.

关 键 词：神经吻合术 补气活血通络法 FOXO3A MAFBX 神经电生理 

分 类 号：R285.5[医药卫生—中药学]