斑蝥黄含量检测方法的改进  

Improvement on Detection Method of Canthaxanthin Content

作　　者：赵红旗 吴曼纯 

机构地区：[1]广州巨元生化有限公司,广东广州510700

出　　处：《广州化工》2017年第13期125-127,共3页GuangZhou Chemical Industry

摘　　要：高效液相色谱法检测斑蝥黄含量所用的流动相由甲醇∶二氯甲烷=85∶15(V∶V)代替乙腈∶甲醇∶二氯甲烷=70∶20∶10(V∶V∶V),改动流动相后斑蝥黄在1.62~16.2μg·mL-1范围内呈良好的线性关系(r=0.9998);平均回收率为99.8%(RSD=0.24%,n=9);重复性试验测得的精密度为0.24%(n=6);检测限为0.81 ng、定量限为1.33 ng。该方法灵敏、准确、专属性好,可用于斑蝥黄的含量测定。The mobile phase used for the determination of canthaxanthin content by high performance liquid chromatography（HPLC） was composed of methanol-dichloromethane（85∶15） instead of acetonitrile-methanol-dichloromethane（70∶20∶10）,after change,the linear range of the method was 1.62-16.2 μg·mL^-1（r=0.9998）,the average recovery was 99.8%（RSD=0.24%,n=9）,RSD of repetitive test was 0.24%（n=6）,the limit of detection and quantitation was 0.81 ng and 1.33 ng,respectively.The established method was sensitive,accurate and specific,which can be used for canthaxanthin determination.

关 键 词：高效液相色谱法 斑蝥黄 含量测定 

分 类 号：O657.72[理学—分析化学]