防风多糖对过敏性鼻炎大鼠免疫因子的影响  

Effect of Polysaccharides from Radix Saplshnikoviae on the Immune Factors in Rats with Allergic Rhinitis

作　　者：耿玉梅[1] 张振巍[1] 石磊[1] 

机构地区：[1]解放军第155中心医院,河南开封475003

出　　处：《中国药师》2017年第7期1188-1191,共4页China Pharmacist

基　　金：济南军区后勤科研计划项目(编号:CJN10L067、CJN14R062)

摘　　要：目的:通过免疫因子水平变化考察防风多糖对卵蛋白致大鼠过敏性鼻炎(AR)模型的治疗作用及其作用机制。方法:采用卵清蛋白(OVA)0.3 mg,Al(OH)_3 30 mg及生理盐水1 ml混合后腹腔注射,进行初次免疫,第15天开始给予4%OVA 200μg滴鼻加强免疫,每天1次,复制AR动物模型。SD大鼠50只随机分为正常组、模型组、阳性组(鼻炎康片组,400 mg·kg^(-1))、防风多糖高、中、低剂量组(600,300,150 mg·kg^(-1))等6组。灌胃给药14 d后,各组大鼠采用腹主动脉取血并分离血清,放免法检测血清免疫因子和炎症因子的含量变化。结果:阳性组和防风多糖剂量组均可不同程度抑制AR导致的喷嚏、搔鼻、流清涕等典型症状,并能减轻相关炎症反应症状。药物干预治疗后,阳性组和防风多糖各剂量组均可下调白介素-4(IL-4)、肿瘤坏死因子(TNF-α)、血管细胞黏附分子(VCAM-1)、白介素-5(IL-5)等细胞免疫因子的表达,提升白介素-12(IL-12)、干扰素-γ(INF-γ)的表达水平,同时抑制炎症因子血清免疫球蛋白E(IgE)、组胺(HA)、白三烯C4(LTC4)、前列腺素D_2(PGD_2)的生成及释放,各剂量组与模型组相比差异均有统计学意义(P<0.05);防风多糖高、中、低三个剂量组量效关系不明显,高剂量组优于中、低剂量组。结论:防风多糖对AR有治疗作用,可下调AR大鼠血清中IL-4、IL-5水平,上调IFN-γ、IL-12水平,调节Th1/Th2淋巴细胞亚群平衡和机体免疫应答,减轻鼻黏膜充血炎症;其减轻OVA致AR过程中免疫因子的生成和释放可能是其治疗AR的重要机制。Objective： To investigate the effects and underlying mechanism of polysaccharides from Radix Saplshnikoviae on the immune factors in rats with allergic rhinitis induced by OVA. Methods： Firstly, 0.3rag OVA, 30mg Al（OH）3 and lml saline were mixed and intraperitoneally injected for the initial immunization, and then 200μg OVA （4%） was given once a day by nasal dripping since the 15th day for the second immunization to establish the allergic rhinitis model. Fifty SD rats were randomly divided into the allergic rhinitis （AR） model group, the polysaccharides from Radix Saplshnikoviae （ 600 mg·kg^-1, 300mg·kg^-1, 150 mg·kg^-1 ） groups, the positive （ Biyankang, 400 mg·kg^-1） group and the normal control group. After the 14-day intragastric administration, blood was collected from abdominal aorta and serum was obtained. The levels of immune factors and inflammatory factors in serum were examined by radioimmunoassay. Results ： The positive group and the polysaccharide groups could inhibit the typical symptoms of allergic rhinitis including sneezing, nasal scratching and running nose in varying degrees, and alleviate the inflammatory reaction symp- toms. After the drug intervention, the positive group and the polysaccharide groups could down-regulate the expression of immune cell factors IL-4, TNF-A, VCAM-1 and IL-5, enhance IL^-12 and INF-γ expression levels, and suppress the generation and release of in-flammation factors IgE, HA, LTC4 and PGD2, and the differences were significant when compared with the model group （P 〈 0.05）. The dose-effect relationship was not too obvious among the polysaccharide from Radix Saposhnikoviae groups respectively at high, medium and low dose, and the high dose group was best among them. Conclusion： The polysaccharides from Radix Saplshnikoviae exhibit curative effect for AR, which can down-regulate the serum levels of IL-4 and IL-5, up-regulate the serum levels of IFN-γ and IL^-12, adjust Th1/Th2 lymphocyte subsets balance and body immune response

关 键 词：防风多糖 过敏性鼻炎 免疫因子 炎症因子 

分 类 号：R285.5[医药卫生—中药学]