高通量低能量激光照射诱导人肺腺癌细胞凋亡过程中survivin的抗凋亡效应的研究  

Mechanism of the inhibition of apoptosis by survivin during high fluence low-power laser irradiation-induced apoptosis

作　　者：储冀汝[1] 沈杰[1] CHU Jiru SHEN Jie(Department of Clinical Pharmacy, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China)

机构地区：[1]皖南医学院弋矶山医院临床药学部

出　　处：《中国临床药理学与治疗学》2017年第6期640-644,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：皖南医学院中青年自然科学基金项目(WK2012F09);安徽省自然科学基金项目(1408085MH163);安徽省高等学校自然科学研究项目(KJ2015A155);国家自然科学基金项目(81641141)

摘　　要：目的:近年来的研究工作表明高通量低能量激光照射(high fluence low-power laser irradiation,HF-LPLI)能诱导细胞凋亡,但survivin是否参与此过程以及它的调节作用尚不清楚。因此,文中主要研究在HF-LPLI处理下,survivin在活细胞中的动态分布以及表达量的变化。方法:应用荧光探针GFP-survivin,在人肺腺癌细胞(human lung adenocarcinoma cells,ASTC-α-1)中实时监测HF-LPLI处理下GFP-survivin的动态行为以及表达量的变化。结果:实验结果表明,通过240 J/cm2的氦氖激光照射,survivin在处理后3 h进入细胞核,并且survivin在细胞内的表达量呈持续升高的趋势。结论:实验结果预示,HF-LPI处理可能调节了肿瘤细胞survivin的表达并且参与调控了某些相关的细胞信号通路蛋白,比如p53、Akt等。进一步的研究将探讨在HF-LPLI处理下,survivin具体调控的转录因子以及调控机制。AIM： Recent years,some studies showed that high fluence low-power laser irradiation（HF-LPLI） can induce apoptosis whereas whether survivin plays an important role in this process and its regulation mechanism is not clear. This article mainly studies the distribution of survivin in nuclear and cytoplasm and the change of expression of survivin under the HF-LPLI treatment. METHODS：The research real-time monitors the dynamic process of survivin under the HF-LPLI treatment with the GFP-survivin in ASTC-α-1 cells. RESULTS： The results showed that survivin translocated to nuclear at 3 hours and the expression quantity of survivin advances with time by LPLI（240 J/cm2）. CONCLUSION：The results implicated that survivin may be regulated by HF-LPLI and regulates some cell signaling proteins,for instance,p53,Akt. The further study will explore that survivin regulate the precise transcription factors and the regulation mechanism.

关 键 词：生存素 细胞凋亡 高通量低能量激光照射 

分 类 号：R965.2[医药卫生—药理学]