MiR-148a在国人血浆中的分布及与高脂血症的关联性探讨  

Relevance of plasma distribution of microRNA-148a in Chinese population and hyperlipidemia

作　　者：李西[1,2] 靳高凤 刘志军[1,2] 李新华[3] 刘名义[1,2] 张红[1,2] 熊玉卿[1,2] LI Xi JIN Gaofeng LIU Zhijun LI Xinhua LIU Mingyi ZHANG Hong XIONG Yuqing(Clinical Pharmacology Institute of Nanchang University Clinical Pharmacokinetics Key Laboratory of Jiangxi Province the Hospital of Nanchang University, Nanchang 330006, Jiangxi, China)

机构地区：[1]南昌大学临床药理研究所 [2]江西省临床药物代谢动力学重点实验室 [3]南昌大学医院

出　　处：《中国临床药理学与治疗学》2017年第6期659-663,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(81260508);江西省"赣鄱英才555工程"人才资助计划(18000046)

摘　　要：目的:探讨国人血浆中microRNA-148a的分布情况,研究健康受试者与高脂血症患者血浆中miR-148a的表达水平的差异性。方法:按临床试验方案收集高脂血症患者血浆样本52例及招募的健康受试者血浆样本77例,采用RT-q PCR测定miR-148a表达水平;以Shapiro-Wilk test对所得实验数据进行正态性检验,两组间采用两独立样本t检验,多组间数据利用one-way ANOVA进行分析。结果:129例受试者血浆中miR-148a表达水平按四分位数分组后,各组的表达水平△Ct分别为1.65±0.80、3.91±0.64、5.68±0.61和8.21±1.16,各组间的差异具有统计学意义(P<0.05);而健康受试者组和高脂血症患者组血浆中miR-148a的表达水平△Ct分别为4.81±2.75和4.84±2.26,两组间差异无统计学意义(P>0.05)。结论:国人血浆中miR-148a的表达水平呈正态分布且各组间的差异具有统计学意义;但健康组与高脂血症患者组血浆中miR-148a的表达差异无统计学意义,提示人体血浆中miR-148a的表达与高脂血症可能无关。AIM： To detect the distribution of miR-148 a level in Chinese people ＇s plasma,and to explore the different expression level of miR-148 a in plasma between healthy subjects and hyperlipidemia patients. METHODS： According to the clinical trial scheme,plasma samples of 52 patients with hyperlipidemia and 77 cases of healthy subjects were collected. RT-q PCR was used to determine the expression of miR-148 a level. Shapiro-Wilk test as a way of normality test to the experimental data. Two independent sample t-test or one-way ANOVA was used to analyze the difference between groups. RESULTS：129 cases of subjects were quartile grouped by miR-148 a level,and the △Ct of miR-148 a were1. 65 ± 0. 80,3. 91 ± 0. 64,5. 68 ± 0. 61 and 8. 21 ±1. 16,respectively. The difference between groups was statistically significant（ P 0. 05）. Whereas,the △Ct of miR-148 a of the healthy subjects group and patients with hyperlipidemia group were 4. 81 ±2. 75 and 4. 84 ± 2. 26,respectively. There was no statistically significant difference between the two groups（ P 0. 05）. CONCLUSION： The expression of miR-148 a in Chinese people＇s plasma is normally distributed,and the difference between four groups is statistically significant. There is no difference between healthy subjects group and patients with hyperlipidemia group, suggesting that miR-148 a may not relate to hyperlipidemia.

关 键 词：miR-148a 表达水平 分布 RT-q PCR 高脂血症 

分 类 号：R589.2[医药卫生—内分泌]