新生儿出生后三天内肠道微生态中菌群分布  

Intestinal microbiota in neonates within three days after birth

作　　者：丁溢芳 肖玲莉[1] 郭佳林[1] 陆炯 徐浩 候美玲 贲晓明[1] 

机构地区：[1]同济大学附属第一妇婴保健院新生儿科,上海201204 [2]上海世纪医学检验所,200093

出　　处：《中华围产医学杂志》2017年第7期507-514,共8页Chinese Journal of Perinatal Medicine

摘　　要：目的 初步探讨新生儿出生后第1天和第3天肠道微生态中菌群分布的特点。 方法 选取2016年6月15日至2016年8月3日在同济大学附属第一妇婴保健院出生的50例单胎、健康新生儿为研究对象。采集新生儿粪便样本，按采集时间分为第1天（记为D1）组和第3天（D3）组，每组各50个。使用Illumina NexSeq高通量测序技术，对样本中所有细菌的16S rRNA基因的V4＋V5区进行DNA测序，获得肠道菌群结构数据。采用Metastats分析法进行统计学分析，并绘制稀释曲线、Shannon-Wiener曲线和群落结构组成柱状图。 结果 （1）100个粪便样本共检测到门25个，科119个，属227个，种159个。（2）2组粪便样本中主要的门均为变形菌门、厚壁菌门、拟杆菌门和放线菌门。与D1组相比，D3组中厚壁菌门、拟杆菌门的相对丰度呈上升趋势（分别为0.27±0.03与0.41±0.05、0.07±0.01与0.09±0.03），放线菌门呈下降趋势（0.10±0.01与0.01±0.00），变形菌门变化不大（0.51±0.03与0.49±0.05）。2组的厚壁菌门、放线菌门的相对丰度比较，差异均有统计学意义（q值均为－0.01，P值均〈0.05）。（3）2组粪便样本中相对丰度前10的共有科为肠杆菌科、葡萄球菌科、肠球菌科、链球菌科和毛螺菌科。与D1组相比，D3组中肠杆菌科、葡萄球菌科、肠球菌科、链球菌科的相对丰度呈上升趋势（分别为0.25±0.02与0.46±0.06、0.07±0.02与0.12±0.03、0.04±0.02与0.10±0.04、0.03±0.02与0.06±0.01），毛螺菌科呈下降趋势（0.03±0.01与0.02±0.02）。2组中只有肠杆菌科相对丰度比较差异有统计学意义（q=0.00，P〈0.05）。（4）2组粪便样本中相对丰度前10的共有属为葡萄球菌属、肠球菌属、链球菌属、拟杆菌属和假单胞菌属。与D1组相比，D3组中寡养单胞菌属、丙酸杆菌属、不动杆菌属、芽孢杆菌属、鞘氨醇单胞菌属等需氧或兼性厌氧菌的相对丰度Objective To investigate the characteristics of intestinal microbiota in neonates on the first and third day after birth. Methods A total of 50 healthy singleton neonates who were born between June 15, 2016 and August 3, 2016 in Shanghai First Maternity and Infant Hospital were enrolled. Their stool samples were collected on the first and third day after birth and the samples were labeled according to the time of collection （D1 and D3 groups, n=50 each）. Illumina NexSeq high-throughput sequencing platform was used to sequence the variable region 4 and 5 of all bacterial 16S rRNA genes in the samples. The composition of intestinal microbial communities was determined and the differences between the two groups were compared by Metastats analysis.Results （1） A total of 100 stool samples were sequenced and the retrieved sequences were from 25 bacterial phyla, 119 families, 227 genera and 159 species. （2） Major phyla in the two groups were the same, namely, Proteobacteria, Frimicutes, Bacteroidetes and Actinobacteria. The relative abundances of Frimicutes （0.27±0.03 vs 0.41±0.05） and Bacteroidetes （0.07±0.01 vs 0.09±0.03） increased over time, while that of Actinobacteria （0.10±0.01 vs 0.01±0.00） decreased on day 3. No significant difference in the relative abundance of Proteobacteria （0.51±0.03 vs 0.49±0.05） was observed between D1 and D3 groups. There were significant difference in relative abundances of Frimicutes and Actinobacteria between the two groups （both q=-0.01, both P〈0.05）. （3） Among the top ten most abundant families, Enterobacteriaceae, Staphylococcaceae, Enterococcaceae, Streptococcaceae and Lachnospiraceae were detected in both of the two groups. The relative abundances of Enterobacteriaceae （0.25±0.02 vs 0.46±0.06）, Staphylococcaceae （0.07±0.02 vs 0.12±0.03）, Enterococcaceae （0.04±0.02 vs 0.10±0.04）, Streptococcaceae （0.03±0.02 vs 0.06±0.01） increased over time, while that of Lachnospiraceae （0.03±0.01 vs 0.02±0.02） decreased

关 键 词：胃肠道 微生物群落 高通量核苷酸序列分析 婴儿 新生 

分 类 号：R722.1[医药卫生—儿科]