99Tcm-3P4-RGD2 SPECT/CT显像评价阿托伐他汀干预后兔动脉斑块稳定性变化的可行性  

Feasibility of 99Tcm-3P4-RGD2 SPECT/CT imaging in evaluation of arterial plaque stability after atorvastatin intervention in rabbits

作　　者：韩洁[1] 张颖[1] 王蒨[1] 米宏志[1] 苏航[1] 牟甜甜[1] 解小芬[1] 李全[1] 张烨虹[1] 

机构地区：[1]首都医科大学附属北京安贞医院、北京市心肺血管疾病研究所核医学科,100029

出　　处：《中华核医学与分子影像杂志》2017年第7期410-414,共5页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金（81271613,81301251）;北京市自然科学基金（7122060）

摘　　要：目的 探讨99Tcm-3P4-RGD2 SPECT/CT显像评价阿托伐他汀干预后兔动脉斑块稳定性变化的可行性.方法 将18只新西兰雄性大白兔按完全随机法分为稳定斑块组（A组）、易损斑块组（B组）、易损斑块他汀干预组（C组）,均以高脂饲料喂养12周,2周时对A组行假手术,B组、C组行腹主动脉拉伤术,同时按体质量予以C组口服阿托伐他汀2.5 mg·kg-1·d-1.各组分别在4、8、12周行99Tcm-3P4-RGD2 SPECT/CT显像,计算T/NT比值.12周末处死实验动物,取腹主动脉行离体显像,以病理及免疫组织化学分析验证,计算各组MVD.组间比较采用单因素方差分析.结果 4周时3组腹主动脉部位均未见明显放射性分布;8周时A、C组腹主动脉放射性摄取稍增高,B组腹主动脉中下段呈条形放射性摄取明显增高.12周时,3组腹主动脉放射性摄取均较8周时增加,以B组增量最为明显;T/NT比值分别为1.579±0.217、1.873±0.226、1.524±0.237（F=8.984,P〈0.05）.12周末离体腹主动脉显像示病变部位放射性分布增高,B组明显高于A组、C组;HE病理染色结果示A组、C组动脉斑块分级为Ⅱ级,B组为Ⅳ级;3组MVD分别为8.17±1.17、15.86±1.07、7.17±1.60,差异有统计学意义（F=90.36,P〈0.05）.结论 99Tcm-3P4-RGD2 SPECT/CT显像能够用于他汀类药物干预后兔动脉易损斑块的稳定性变化的效果评价,具有较高的灵敏度.Objective To investigate the feasibility of a novel molecular probe 99Tcm-3P4-RGD2 in evaluating arterial plaque stability after atorvastatin intervention in rabbits with SPECT/CT. Methods Eighteen male New Zealand rabbits were randomly divided into group A （stable plaque）, group B （vulnerable plaque）, and group C （vulnerable plaque with statin intervention）. All rabbits were fed with high-fat food for 12 weeks. After high-fat feeding for two weeks, sham surgery was performed on group A. In the meantime, abdominal aorta injury was performed on group B and group C. After that, rabbits of group C were given oral atorvastatin （2.5 mg·kg-1·d-1）. 99Tcm-3P4-RGD2 SPECT/CT imaging was performed on each group at the end of 4, 8 and 12 weeks. T/NT ratios were calculated. Animals were sacrificed at the end of 12 week after imaging studies. The abdominal aortas were collected, imaged with SPECT/CT, and evaluated by pathological HE staining and immunohistochemical analysis. MVD was calculated. Differences among 3 groups were analyzed using one-way analysis of variance. Results There was no significant radioactive uptake in the abdominal aortas of three groups on the 4th week′s imaging. The radioactive uptake in abdominal aortas increased slightly on the 8th week, with the highest radioactive uptake in group B. The radioactivity in abdominal aortas of the 3 groups maintained increasing on the 12th week, with T/NT ratios of 1.579±0.217, 1.873±0.226 and 1.524±0.237, respectively （F=8.984, P〈0.05）. In ex vivo abdominal aorta images, especially images of group B, radioactivity in lesion sites was higher than that in normal tissue. Accordingly, results of HE staining showed that artery plaques of group A and group C were grade Ⅱ and group B was grade Ⅳ. The MVD of group A, B and C was 8.17±1.17, 15.86±1.07 and 7.17±1.60, respectively （F=9036, P〈0.05）. Conclusion 99Tcm-3P4-RGD2 SPECT/CT imaging has a high sensitivity in the evaluation of arterial plaque stability after statin intervention

关 键 词：动脉粥样硬化性 阿托伐他汀钙 体层摄影术 发射型计算机 单光子 体层摄影术 X线计算机 精氨酸-甘氨酸-天冬氨酸 兔 

分 类 号：R543.3[医药卫生—心血管疾病] R817.4[医药卫生—内科学]