希森美康XN-1000血细胞分析仪白细胞分类与人工涂片分类结果的比较分析  

Sysmex XN-1000 hematology analyzer in accuracy of the white blood cell differential count is different from artificial blood smear examination

作　　者：郝瑞春[1] 张雅蓉[1] 

机构地区：[1]鄂尔多斯市中心医院检验科,内蒙古鄂尔多斯017000

出　　处：《实用检验医师杂志》2017年第2期96-98,共3页Chinese Journal of Clinical Pathologist

摘　　要：目的评价希森美康XN-1000血细胞分析仪(仪器法)对静脉血白细胞分类计数的准确性,为临床标本检查的规范化提供依据。方法选择120份用仪器法检测血常规结果出现白细胞分类计数有报警提示的标本,同时进行人工涂片分类(镜检法)复检,比较仪器法和金标准镜检法的结果。结果仪器法与镜检法比较,白细胞计数分类中,中性粒细胞与单核细胞分类两组存在显著性差异(P=0.000),仪器法检测存在单核细胞假性升高,中性粒细胞假性减低;仪器法和镜检法符合率是69.17%(阳性标本83份),仪器法假阳性率是30.83%(37份假阳性)。结论希森美康XN-1000血细胞分析仪白细胞出现报警提示可以有效筛选阳性标本,但同时对阳性标本需要进行人工镜检复核,以避免假阳性结果。Objective Study on the Sysmex xn 1000 hematology analyzer in accuracy of the white blood cell differential count in venous blood. Methods Compared the white blood cell differentials provided （or abnormal cells detected） by the automated hematology analyzer XN-1000 （instrumental method） in 120 blood routine examinations （Blood RE） with those obtained through a corresponding artificial blood smear examination （microscopic method） by cytologists. Results There was a significant difference between instrumental method and microscopic method in monocyte count and neutrophil granulocyte count （P = 0.000）, instrumental method showed pseudo- high results in monocyte count and pseudo-low results in neutrophil granuhcyte count. The coincidence rate of instrumental method and microscopic method is 69%, and the false positive rate was 31%. Conclusions Sysmex XN-IO00 hematology analyzer is an efficient method to select positive samples （analyzer generated flags when detected abnormal ceils）, while a manual microscopic film examination of the corresponding positive samples remains necessary for avoiding false positive results.

关 键 词：XN-1000型血细胞分析仪 白细胞分类 人工涂片分类 

分 类 号：R446.1[医药卫生—诊断学]