Box-Behnken设计-效应面法优选清咽爽喉颗粒醇沉工艺研究  

Optimization of alcohol precipitation process for Qingyan Shuanghou granules using Box-Behnken design-response surface methodology

作　　者：李永祝[1] 文远大[1] 余俊[1] 彭平健 杨贤军[1] 

机构地区：[1]湖南省怀化市第一人民医院,湖南怀化418000

出　　处：《湖南中医杂志》2017年第6期165-167,共3页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药管理局资助项目(编号:201586)

摘　　要：目的:优选清咽爽喉颗粒最佳醇沉工艺参数。方法:采用Box-Behnken设计-效应面法对清咽爽喉颗粒进行醇沉工艺的研究,以浸膏得率、绿原酸提取量为评价指标,采用多指标综合加权评分的方法优选出最佳醇沉工艺,并对优选工艺进行验证。结果:最佳醇沉工艺为浓缩液1.15(60℃测),加入乙醇使含醇量达到70%,放置时间24h。结论:优选的清咽爽喉颗粒醇沉工艺合理可行,可为清咽爽喉方的工艺研究提供参考。Objective：To investigate the optimal alcohol precipitation process parameters for Qingyan Shuanghou gran- ules. Methods：Box -Behnken design -response surface methodology was used to analyze the alcohol precipitation process for Qingyan Shuanghou granules. With extraction rate and chlorogenie acid extraction yield as assessment indices, a multi - index and comprehensiveweighted grading method was used to determine the optimal alcohol precipitation process, and then the optimal process was validated. Results：The optimal alcohol precipitation process was concentrated solution 1.15 （measured at 60℃ ） , addition of alcohol to 70% , and a storage time of 24 hours. Conclusion：The optimized alcohol precipitation process for Qingyan Shuanghou granule is rational and feasible and can provide a reference for process studies on Qingyan Shuanghou prescription.

关 键 词：清咽爽喉颗粒 醇沉工艺 效应面法 实验研究 

分 类 号：R284.1[医药卫生—中药学]