人参皂苷Rg1通过调控骨骼肌TRB3/AKT信号通路改善大鼠胰岛素抵抗的分子机制研究  

The Molecular Mechanism of Ginsenoside Rg1 Alleviates Insulin Resistance by Regulating the TRB3/AKT Signal Pathway

作　　者：郑永仁[1] 王礴[1] 刘传凤 杨晓密[1] 

机构地区：[1]云南中医学院科研实验中心,云南昆明650500 [2]云南白药集团股份有限公司,云南昆明650500

出　　处：《中国民族民间医药》2017年第13期33-37,40,共6页Chinese Journal of Ethnomedicine and Ethnopharmacy

基　　金：云南省教育厅科学研究基金一般项目(NO:2014Y245)

摘　　要：目的:探讨人参皂苷Rg1对大鼠骨骼肌的保护作用,并讨论其通过调控骨骼肌TRB3/AKT信号通路缓解大鼠IR的分子机制。方法:50只雄性SD大鼠随机分为5组,正常对照组给予普通饲料喂养,胰岛素抵抗(IR)组以及人参皂苷Rg1低剂量治疗组、中剂量治疗组以及高剂量治疗组给予高脂饲料喂养,4周后,分别对Rg1低剂量、中剂量和高剂量治疗组大鼠予以Rg1 25 mg/kg·d、50 mg/kg·d和100 mg/kg·d灌胃治疗,同时IR组大鼠予以相同体积生理盐水灌胃,4周后处死大鼠,收集血清和骨骼肌组织。分别采用ELISA、RT-q PCR和Western blot等方法进行相关因子检测。结果:与对照组比较,IR组大鼠血清中TG、TC、LDL-C、HOMA-IR、MDA、TNF-α、IL-6和IL^(-1)β含量升高(P<0.05),人参皂苷Rg1治疗后,上述指标下降(P<0.05)且具有剂量依赖性;SOD和GSH的含量降低,人参皂苷Rg1治疗4周后升高(P<0.05);且骨骼肌组织中TRB3的mRNA和蛋白表达水平,经人参皂苷Rg1治疗后下降明显(P<0.05),AKT(Ser473位点)的磷酸化水平升高(P<0.05),且都具有剂量依赖性。结论:人参皂苷Rg1可以降低机体HOMA-IR、减轻炎症和氧化应激反应、改善血脂,并通过调控骨骼肌TRB3/AKT信号通路保护大鼠骨骼肌代谢,缓解IR。Objective To investigate the effect of ginsenoside Rgl protect the skeletal muscle of rats, and discuss the molecular mechanism of Rgl ameliorates the insulin resistance through the TRB3/AKT signal pathway. Methods 50 male SD rats were divided into five groups randomly. The rats in control group were feed on an ordinary chow, and the rats in IR, LD Rgl, MD Rgl, and HD Rgl groups were fed with a high - fat diet. After 4 weeks, the rats of treatment groups were treated with ginsenoside Rgl （25 mg/kg·day, 50 mg/kg·day and 100 mg/kg·day） by gavage respectively, while the rats of IR group received normal saline with the same method, all of the rats were sacrificed after 4 weeks, the serums and skeletal muscle tissues were collected. The related factors were determined by ELISA, RT - qPCR and Western blott respectively. Results Compared with the control group, the concentrations of TG, TC, LDL - C, HOMA - IR, MDA, TNF -α, IL - 6 and IL - 1β in the serum of the rats of the IR group were upregnlated, and downregnlated obviously after Rgl treatment for 4 weeks （ P 〈 0. 05 ）, the concentrations of GSH and SOD in the serum of the rats of the IR group were downregu-lated, and increased after Rgl treatment （P 〈 0. 05 ） ; Compared with the control group, the expression levels of mRNA and protein of TRB3 in the skeletal muscle tissues of IR group were increased （ P 〈 0. 05 ） , after Rgl adminisration for 4 weeks, the expression levels of TRB3 were decreased obviously （ P 〈 0. 05 ） , the phosphorylation levels of AKT were upregnlated （ P 〈 0. 05 ）, the effect of ginsenoside Rgl has a dose- dependent manner. Conclusion Ginsenoside Rgl is capable to alliviate the HOMA -IR, dyslipidemia, inflammatory and oxidative stress and ameliorate the IR by regulating the the TRB3/AKT signal pathway in skeletal muscle.

关 键 词：人参皂苷RG1 胰岛素抵抗 骨骼肌 TRB3 AKT 

分 类 号：R285.5[医药卫生—中药学]