2个玉米光敏色素C基因的转录丰度对多种光质处理的响应  

Transcription Abundances of Two Phytochrome C in Response to Different Light Treatments in Zea mays

作　　者：牛骧 郭林[2] 杨宗举 孙蕾[2,3] 李红丹[2,3] 游光霞[2] 徐宏[4] 孟凡华[2] 佘跃辉[1] 杨建平[2] NIU Xiang GUO Lin YANG ZongJu SUN Lei LI HongDan YOU GuangXia XU Hong MENG FanHua SHE YueHui YANG JianPing(College of Agronomy, Sichuan Agricultural University, Chengdu 611130 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beoing 100081 Graduate School, Chinese Academy of Agricultural Sciences, Beo＇ing 100081 Huangchuan Institute of Agricultural Sciences of Henan Province, Huangchuan 465150, Henan)

机构地区：[1]四川农业大学农学院,成都611130 [2]中国农业科学院作物科学研究所,北京100081 [3]中国农业科学院研究生院,北京100081 [4]河南省潢川县农业科学研究所,河南潢川465150

出　　处：《中国农业科学》2017年第12期2209-2219,共11页Scientia Agricultura Sinica

基　　金：国家转基因生物新品种培育科技重大专项(2016ZX08010002-003-002);国家重点研究发展计划(2016YFD0101002);北京市自然科学基金(重点)(6151002);中国农业科学院科技创新工程

摘　　要：【目的】通过NCBI数据库获得2个玉米PHYC及相关数据,并进行相关生物信息学分析。利用实时荧光定量PCR(q RT-PCR)分析2个玉米PHYC在玉米各器官的转录丰度,以及其转录丰度对多种光质处理、黑暗到各种光质转换和光周期处理(长日照和短日照)的响应,为研究玉米PHYC在玉米幼苗去黄化与开花期的调控机制奠定基础。【方法】采用玉米B73自交系为研究材料,通过RT-PCR分别对Zm PHYC1和Zm PHYC2的全长ORF序列进行克隆;借助相关软件对其进行生物信息学分析,利用q RT-PCR分析这两个基因在玉米各器官中的转录丰度,及其转录丰度对各种光照处理的响应。【结果】Zm PHYC1和Zm PHYC2的全长ORF均为3 408 bp,编码1 135个氨基酸基序,分子量分别为126.14和126.07 k D。生物信息学分析表明,玉米phy C蛋白可以分为6个功能区段:节奏周期蛋白—Ah核转运接受蛋白—专一蛋白区段(Per-Arnt-Sim,PAS)、c GMP受激磷酸二酯酶区段(GAF)、色素区段(PHY)和PAS相关区段(PRD,包含2个PAS区段)、组氨酸激酶A区段和组氨酸激酶ATP酶区段,但是Zmphy C2在PRD区段仅有一个PAS区段。氨基酸水平的系统发育树分析表明,Zmphy C1和Zmphy C2与禾本科物种phy C有很高的一致性,且与甘蔗和高粱phy C的亲缘关系较近。q RT-PCR分析表明,Zm PHYC1和Zm PHYC2的表达在根和叶中的转录丰度均较高,同时对持续蓝光和白光响应强烈;在黑暗到各种光质转换处理中,这两个PHYC的表达模式相似。在黑暗转到远红光、红光、蓝光和白光的0.5 h,Zm PHYC1和Zm PHYC2的转录表达均急剧上升,随后迅速下降到自身起始黑暗时的水平以下,并上下波动。这两个基因对长日照和短日照的光周期处理也能积极响应,在长日照条件下,2个Zm PHYC出现了极其相似的表达模式,均在光照和黑暗阶段各出现1个峰值;在短日照条件下,这两个基因的表达模式差异较大,Zm PHYC1的峰值出现在进入黑暗[Objective] To study the functions of phytochrome C genes in seedling de-etiolation and flowering regulation in maize （Zea rnays L.）, two phytochrome C genes of maize （ZmPHYC1 and ZmPHYC2） were selected from the NCBI database and analyzed by bioinformatic methods. The transcription abundances of two ZmPHYC genes was analyzed in different tissues and in response to light qualities, transitions from the dark to different light conditions, photoperiod treatment （long day and short day） by quantitative RT-PCR （qRT-PCR）. [Method] B73 inbred line was used in this study, the full length ORFs of two ZmPHYC genes were cloned by RT-PCR. The proper clones were sequenced and analyzed by bioinformatics software. The transcription abundances of two ZmPHYC genes in different tissues and in response to light treatments was detected using qRT-PCR. [ Result] Both the full length ORFs of ZrnPHYC1 and ZmPHYC2 contained 3408 nucleotides and encoded two polypeptides with 1135 amino acid motifs, and their molecular weight was 126.14 kD and 126.07 kD, respectively. Two ZmphyC proteins were able to be further divided into six domains： Per （period circadian protein）-Arnt （Ah receptor nuclear translocator protein）-Sim （single-minded protein） （PAS）, cGMP-stimulated phosphodiesterase （GAF）, phytochrome （PHY）, PAS-related domain （PRD） containing two PAS, His Kinase A domain （HisKA）, Histidine kinase-like ATPases （HATPase_c）, while ZmphyC2 lacked a PAS in PRD domain. Phylogenetic analysis indicated that the two ZmphyC proteins belonged to the same branch with phyC proteins from other graminaceous species, especially with the phyC proteins from sugarcane and sorghum, qRT-PCR assays showed that both ZmPHYC1 and ZmPHYC2 belonged to tissue-specific genes and highly expressed in roots and leaves. The transcription abundances of the both genes were very high under blue and white light conditions. Both ZmPHYC1 and ZrnPHYC2 displayed similar expression patterns during transitions from the dark to d

关 键 词：玉米 Zm PHYC 光信号转导 表达模式 光质处理 

分 类 号：S513[农业科学—作物学]