中国荷斯坦牛CXCR1基因编码区SNP多态与临床乳房炎和生产寿命的关联分析  

Association Between SNPs in the CDS Regions of CXCR1 Gene and the Clinical Mastitis and Lifetime for Chinese Holstein

作　　者：王梦琦[1] 倪炜[1] 张慧敏[1] 杨章平[1] 王西朴 蒋彦森 毛永江[1,2] WANG MengQi NI Wei ZHANG HuiMinI YANG ZhangPing WANG XiPu JIANG YanSen MAO YongJiang(College of Animal Science and Technology, Yangzhou University, Yangzhou 225009 Jiangsu Junlebao Dairy Co., Ltd. Xuzhou 221721, Jiangsu)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏君乐宝乳业有限公司,江苏徐州221721

出　　处：《中国农业科学》2017年第12期2359-2370,共12页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31372286);江苏省优势学科(PAPD);江苏省企业研究生工作站资助

摘　　要：【目的】探讨中国荷斯坦牛CXCR1基因编码区SNP突变与临床乳房炎和生产寿命的相关性。【方法】根据CXCR1基因编码区序列,利用PCR-直接测序法对低SCS和高SCS样本各20个样本进行SNP筛查,最后对所选择的4个SNP位点利用飞行质谱法对866头中国荷斯坦牛进行检测,同时收集所检测牛只临床乳房炎和生产寿命等信息,利用多因素方差分析法、Logistic回归、Cox生存回归等方法分析以上SNP位点突变与临床乳房炎发生次数和生产寿命的相关性。【结果】CXCR1基因编码区共发现13个SNP位点(291 C>T、333 C>G、337 A>G、365 C>T、570 A>G、642 A>G、735 C>G、816 A>C、819 A>G、980 A>G、995 A>G、1008 C>T和1068 A>G),分为4个连锁群,随后从每个连锁群中各选择1个SNP位点(642 A>G,816A>C,980 A>G和1068 A>G),利用飞行质谱法对大样本中国荷斯坦牛进行检测。4个SNP位点共有9种单倍型,其中单倍型GAGG频率最高(0.3141),而单倍型ACAA频率最低(0.0017)。CXCR1-642与2胎牛患临床乳房炎次数有显著相关(P<0.05),AG基因型个体2胎奶牛患临床乳房炎次数显著高于AA基因型(P<0.05),CXCR1-816 AA基因型个体3胎奶牛患临床乳房炎次数显著低于AC和CC基因型(P<0.05),其它SNP位点与各胎次临床乳房炎发生次数均无显著相关(P>0.05)。CXCR1-816与奶牛离群月龄有极显著相关(P<0.01),与奶牛生产月龄和离群胎次有显著相关(P<0.05),CXCR1-816 AA基因型个体生产月龄、离群月龄和离群胎次均显著高于CC基因型个体(P<0.05),而其它SNP位点对生产月龄、离群月龄和离群胎次均无显著相关(P>0.05)。Cox生存分析表明:只有CXCR1-816位点与奶牛生存时间有显著相关(P<0.05),CXCR1-816 CC基因型个体在各时间段的生存概率均低于AA和AC基因型个体。【结论】CXCR1-816 A>C突变与中国荷斯坦牛患临床乳房炎次数和生产寿命有显著相关,在进一步验证其功能后,可用于中国荷斯坦牛生产寿命的分子标[Objective] The objective of this study was to investigate_the association between the SNPs in the coding sequence （CDS） of CXCR1 gene and the clinical mastitis and lifetime for Chinese Holstein. [Method] SNPs in the CDS of CXCR1 gene were screened by using PCR and direct sequencing for 20 cows with low SCS and 20 cows with high SCS. Finally, the selected SNPs of CXCR1 gene for 866 Chinese Holstein cows were detected using flight mass spectrometry. The clinical mastitis and productive life of tested cows were collected from the management system of dairy farm. The association between the SNPs and the clinical mastitis and productive life of tested cows was analyzed using multi factor variance analysis, Logistic regression, Cox regression. [Result] A total of 13 SNPs were found in the CDS region of CXCR1 gene （291 C^T, 333 C〉G, 337 A〉G, 365 C^T, 570 A〉G, 642 A^G, 735 C^G, 816 A〉C, 819 A^G, 980 A^G, , 995 A^G, 1008 C^T and 1068 A〉G）, and these SNPs were divided into 4 linkage groups, and four SNPs were selected from each linkage group for further analysis （642 A^G, 816 A^C, 980A^G and 1068 A^G）. A total of 9 haplotypes were observed for 4 SNPs ofCXCR1 gene, the GAGG haplotype frequency was the highest （0.3141）, and the frequency of haplotype ACAA was the lowest （0.0017）. CXCR1-642 A^G showed a significant association with the number of dairy cows suffering from clinical mastitis in second lactation （P~ 0.05）. The number of dairy cows suffering from clinical mastitis for individual with AG genotype was significantly higher than that of AA genotype （P〈 0.05）. The number of dairy cows suffering from clinical mastitis for individual with CXCR1-816 AA genotype was significantly higher than that of AC and CC genotypes （P〈0.05）. The other SNPs showed no significant association with the number of clinical mastitis. CXCR1-816 A^C mutation showed an extremely significant association with culling age （P〈0.01） and a significant association with productive month and culling la

关 键 词：中国荷斯坦牛 CXCR1 SNP 临床乳房炎 生产寿命 

分 类 号：S823[农业科学—畜牧学]