灯盏花通过促进eNOS/NO合成减轻缺血再灌注引起的大鼠肠道黏膜屏障损伤  

Breviscapine pretreatment attenuates ischemia reperfusion induced intestinal mucosal barrier damage via promoting eNOS/NO production

作　　者：孙孟彪[1] 张政[2] 韩雪[3] 张献玲[4] 

机构地区：[1]北京大学深圳医院麻醉科,广东省深圳市518035 [2]中山大学附属第三医院麻醉科,广州市510630 [3]中山大学孙逸仙纪念医院麻醉科,广州市510120 [4]中山大学附属第三医院外科,广州市510630

出　　处：《实用医学杂志》2017年第13期2122-2125,共4页The Journal of Practical Medicine

基　　金：深圳市科技计划资助项目(编号:JCYJ20140415162542986)

摘　　要：目的探讨灯盏花在肠缺血再灌注(I/R)早期小肠黏膜屏障受损中的作用及其机制。方法 44只SD大鼠随机分为假手术组、肠I/R损伤(IIR)组、灯盏花处理(EB+IIR)组、L-NAME处理(LN+IIR)组。EB+IIR组在术前7 d腹腔注射灯盏花注射液20 mg(/kg·d),LN+IIR组在术前30 min尾静脉给予L-NAME(100mg/kg)或等量生理盐水。夹闭肠系膜前动脉45 min后再灌注4 h。处死大鼠取标本,HE染色观察肠组织病理改变,ELISA检测肠黏膜SIgA和血浆内毒素水平,同时检测肠组织iNOS、eNOS和总NO表达水平。结果肠I/R导致明显小肠机械屏障和免疫屏障受损,表现为肠黏膜SIgA含量下调,血浆内毒素水平提高,eNOS含量减少,iNOS/NO合成增加;灯盏花预处理可促进eNOS/NO合成,下调iNOS水平,减轻肠道损伤,提高肠黏膜SIgA含量并减轻血浆内毒素水平;当应用L-NAME HCI后,灯盏花的保护作用被取消。结论灯盏花注射液预处理通过促进eNOS/NO合成减轻I/R引起的小肠损伤。Objective To investigate the effects of breviscapine injection on intestinal mucosal barrier damage induced by intestine ischemia-reperfusion （IIR）. Methods 44 old SD rats were randomly divided into four groups： sham, intestine ischemia-reperfusion （IIR）, EB＋IIR, LN＋IIR. Breviseapine injection 20 mg/（kg, d） was given intraperitoneally in EB＋IIR group. L-NAME （100 mg/kg） was given intravenously 30 rain before surgery in LN＋IIR group. Rats were subjected to superior mesenteric artery occlusion consisting of 45 rain of ischemia and 4 h of reperfusion ; sham laparotomy served as controls. Intestine pathology was assayed by H＆E staining. Concentrations of SIgA, iNOS, eNOS and NO in intestinal mueosa, also endotoxine in plasma, were determined by ELISA. Results IIR induced serious intestinal mechanical and immune barrier damage, evidenced as poor intestine pathology, depression of intestinal SIgA and eNOS levels, elevation of intestinal iNOS/NO levels. However, breviscapine injection pretreatment could promote eNOS/NO production, down-regulated iNOS expression, leading to elevating SIgA concentration in intestine, attenuate endotoxemia induced by IIR. The protection was canceled when application of L-NAME. Conclusion Breviscapine pretreatment attenuates ischemia-reperfusion-induced intestinal mucosal barrier damage via promoting eNOS/NO production.

关 键 词：灯盏花注射液 肠缺血再灌注 分泌型免疫球蛋白A 内毒素血症 一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]