稳定同位素技术在土壤N2O溯源研究中的应用  

A review on development of stable isotope technique in the studies of N_2O formation mechanism

作　　者：林伟[1] 房福力[1] 张薇[1] 丁军军[1] 李玉中[1,2] 徐春英[1] 李巧珍[1] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,农业部旱地节水农业重点实验室,北京100081 [2]中国农业科学院环境稳定同位素实验室,北京100081

出　　处：《应用生态学报》2017年第7期2344-2352,共9页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(41473004,41501318,41301553)资助~~

摘　　要：氧化亚氮(N_2O)作为三大温室气体之一,追溯其来源已经成为研究热点,稳定同位素技术在土壤N_2O来源追踪方面已得到广泛应用.本文概述了产生N_2O的微生物、产生过程及其主要影响因素,综述了国内外关于利用N_2O同位素特征值δ^(15)N、δ^(18)O和SP值(N_2O分子内^(15)N在不同位置上的位嗜值)分析其来源的研究进展,以期为今后相关研究提供参考.其中关于SP的研究在国内尚属初级阶段,因此本文重点介绍国外SP在其溯源上的研究情况,并对今后国内相关研究提出一点看法.As one of three major greenhouse gases, nitrous oxide （ N2 O） has solicited substantial attention. Stable isotope has been widely used to explore the sources of N20 emissions. Here, we briefly introduced the microbial processes involved in N20 emissions, and the main influencing fac- tors. We further summarized the development of N20 isotope signature of δ15N, δ18O and SP （site preference of 15N in different positions of N20 molecule） in exploring the N20 formation mecha- nism. The application of these techniques, especially the SP values, is still at the primary stage in China. Therefore, this paper focused on the development of the isotope ratios analysis in partitioning N20 sources from foreign countries, and put forward suggestions on the future research in China. Key words： stable isotope ; soil microbe ; nitrification ; denitrification; site preference （SP）.

关 键 词：稳定同位素 土壤微生物 硝化作用 反硝化作用 位嗜(SP) 

分 类 号：S154.1[农业科学—土壤学]