Box-Behnken响应面分析法优化桦褐孔菌多糖提取工艺  

Optimization of Extraction Process for Polysaccharide from Inonotus Obliquus by Box-Behnken Response Surface Methodology

作　　者：蔡丽[1] 陈忠敏[1] 王富平[1] 袁森[1] 阮廷飞 

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《重庆理工大学学报（自然科学）》2017年第6期113-119,共7页Journal of Chongqing University of Technology：Natural Science

摘　　要：根据Box-Behnken试验设计原理,在单因素实验基础上,研究料液比、提取温度、提取时间、酶解时间及其交互作用对多糖得率的影响。结果表明:桦褐孔菌多糖的最优提取工艺为:料液比1∶30,提取温度90℃,提取时间5 h,酶解时间65 min,多糖得率理论值为3.598%,实际测得多糖得率为(3.575±0.24)%。Box-Behnken实验设计方法可较好地对桦褐孔菌多糖的提取工艺进行优化,在该工艺下提取得到的桦褐孔菌多糖质量稳定。This study aimed to obtain the optimum extraction process of polysaccharide from Inonotus Obliquus. The response surface methodology （RSM） was used to optimize the extraction process. Liquid/solid ratio, extraction temperature, extraction time, enzymatic hydrolysis time were selected as independent variables on the basis of single factor experiments. The yield of polysaccharides was chosen as the response variable. An extraction condition with solid-liquid ratio 1：30, extraction temperature 90℃, extraction time 5 h and enzymatic hydrolysis 65 min is optimal. The extraction yield under the optimized conditions is （ 3. 575 ± 0.24 ） %, agreeing with the predicted value. The results indicated that this method can rationally optimize extraction process of polysaccharide from Inonotus Obliauus.

关 键 词：桦褐孔菌 多糖 Box-Behnken响应面分析法 

分 类 号：R284.2[医药卫生—中药学]