氨基化荧光碳点制备及其荧光探针构建  

Preparation and evaluation of fluorescent carbon dots(CDs) and CDs based immunofluorescence probe

作　　者：张春林[1] 张勇 车望远 李钒[3] 房玉海 洪志飞[1] 刘长军[3] 

机构地区：[1]东丽医院消化内科,天津300300 [2]北京房山区良乡医院风湿科,北京102443 [3]军事医学科学院卫生装备研究所,天津300161

出　　处：《医疗卫生装备》2017年第6期22-26,共5页Chinese Medical Equipment Journal

基　　金：国家自然科学基金青年项目(51303210;51502345);天津市应用基础与前沿技术研究计划一般项目(14JCYBJC29500)

摘　　要：目的 :依据抗原-抗体免疫荧光分析原理,构建荧光碳点-大肠杆菌抗体免疫荧光探针。方法 :选取富含氨基的氨基葡萄糖为碳源,通过微波加热法制备荧光碳点,再通过EDC/NHS化学偶联法与大肠杆菌抗体结合,形成免疫荧光探针。利用荧光光谱仪、透射电子显微镜、红外光谱仪、紫外-可见光吸收光谱仪等评价碳点以及荧光探针的理化性能。结果:制备的碳点具有优异荧光性能与水溶性,构建的免疫荧光探针可实现大肠杆菌快速检测。结论:碳点是一种环境友好、荧光性能稳定的新型纳米功能材料,有望成为一种优秀的检测试剂。Objective To fabricate an immunofluorescence probe system of carbon dots conjugated antibody based on antigen-antibody reaction principles. Methods A green one-step microwave assisted pyrolysis method was applied to preparing fluorescent carbon dots（CDs） using aminoglucose as carbon source and the obtained CDs were conjugated with antibody via EDC/NHS reactions to build CDs based fluorescent probe. Furthermore, the properties of CDs and CDs based probe system were evaluated by Fourier transform infrared（FTIR） spectra, transmission electron microscopy（TEM）, UV-vis absorption and so on. Results The as-prepared CDs showed excellent fluorescence and hydrophilicity and CDs based immunofluorescence probe exhibited the capability of quick detection of E.coli O157∶ H7. Conclusion Fluorescent CDs as one new emerging environment-friendly nanomaterial has great potential in biosensors.

关 键 词：碳点 荧光 免疫荧光探针 纳米材料 大肠杆菌 

分 类 号：R392-33[医药卫生—免疫学] TB383.1[医药卫生—基础医学]