一株粘细菌Myxococcus sp.STXZ90的分离鉴定及生物活性分析  

On Isolation,Identification and Bioactivitiy of Myxococcus sp.STXZ90

作　　者：黄同龙[1] 雷良欢 廖继燕 周偲[1] 吴雨婧[1] 李纯[1] 魏慧[1] 夏立秋[1] 丁学知[1] 张友明[1] HUANG Tong-long LEI Liang-huan LIAO Ji-yan ZHOU Cai WU Yu-jing LI Chun WEI Hui XIA Li-qiu DING Xue-zhi ZHANG You-ming＂(State Key Laboratory Breeding Base of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, China)

机构地区：[1]湖南师范大学生命科学学院,微生物分子生物学国家重点实验室培育基地,中国长沙410081

出　　处：《湖南师范大学自然科学学报》2017年第2期25-32,共8页Journal of Natural Science of Hunan Normal University

基　　金：国家国际合作资助项目(2011DFA32610);国家“973”计划专项资助项目(2012CB722301);国家“863”计划资助项目(2011AA10A203);湖南省协同创新中心资助项目(20134486)

摘　　要：采用灭活大肠杆菌诱导法分离粘细菌,通过形态观察、生理生化实验、16S rRNA基因序列同源性分析,对其进行鉴定并归类.并通过平板对峙法、肿瘤细胞毒性实验等检测粘细菌代谢产物的抑菌和抗肿瘤活性,RPHPLC分离抗肿瘤活性物质.分离得到一株粘细菌,鉴定结果表明该菌株为黄色粘球菌,将其命名为Myxococcus sp.STXZ90(简写为STXZ90).抑菌试验表明,STXZ90对多种人类病原菌及鱼类致病细菌如产气肠杆菌、嗜水气单胞菌等具有较高的抑制活性;STXZ90发酵上清的甲醇粗提物对多种肿瘤细胞具有广谱高效的抑制肿瘤细胞活性,对Hep-3B,Hela和HUVEC等的最低半抑制浓度(IC_(50))为0.279~1.228 mg/L.对该甲醇粗提物进行RP-HPLC分离,得到2个较纯的单峰物质,体外肿瘤细胞实验结果显示该组分有较强的抑制肿瘤细胞活性.The inactivated Escherichia. coli-dependent fruiting baiting technique was used to isolate myxobacterial strains. Based on the phylogenetical characteristics, physiological and biochemical properties and 16S rRNA gene sequence homology analysis, the isolated strain has been identified and classified. The inhabitation of the strain to different pathogens and tumor cells were detected by the Plate Confrontation Culture Method and tumor cell toxicity experiment. The antitumor substance was further purified by RP-HPLC. Our results showed that the strain belonged to myxococcus xanthus and thus it was named as Myxococcus sp. STXZ90 （STXZ90）. The antibacterial ex- periment indicated that STXZ90 had bacteriostatic activity to different human pathogens and fish pathogens, such as Enterobacter aerogenes, and Aeromonas hydrophil. The methanol elution of the fermented supernatant of STXZ90 exhibited a broad spectrum and high effect on various tumor cell lines. The ICso （half maximal inhibitory concentration） to Hep-3B, Hela, and HUVEC were between 0. 279 to 1. 228 mg/L. The methanol elution was further purified by RP-HPLC with 2 single peak fragments obtained, which displayed strong inhibitory activities to cancer cells.

关 键 词：粘细菌 次级代谢产物 高效液相色谱 

分 类 号：Q939[生物学—微生物学]