不同浓度同型半胱氨酸对内皮细胞TRPV1蛋白表达的影响  

Effects of Homocysteine with Different Concentrations on the Expression of TRPV1 in Endothelial Cells

作　　者：杨绍兵[1,2] 蔡庆 王凯[2] 杜海林[2] 燕茹[4] 丛广志[4] 贾绍斌[4] 

机构地区：[1]宁夏医科大学总医院,银川750004 [2]宁夏医科大学,银川750004 [3]宁夏银川市妇幼保健院妇保科,银川750001 [4]宁夏医科大学总医院心血管内科,银川750004

出　　处：《宁夏医科大学学报》2017年第4期399-402,F0004,共5页Journal of Ningxia Medical University

基　　金：宁夏医科大学优势学科群建设科研项目(XY201501)

摘　　要：目的研究不同浓度同型半胱氨酸对内皮细胞TRPV1蛋白表达水平和细胞增殖的影响。方法将人脐静脉内皮细胞分别用不同浓度同型半胱氨酸(0、0.05、0.1、0.2、0.5、1、2、4、8和16mmol·L^(-1))及不同时间(6、12、24、36和48h)进行干预后用CCK-8检测细胞增殖及活性情况,选择合适的干预浓度进行下一步干预分组(0、0.1、0.5、1和2mmol·L^(-1)),Western blot检测TRPV1蛋白表达水平。结果 1)同型半胱氨酸对人脐静脉内皮细胞的增殖能力有抑制作用,光密度(OD)值从(1.03±0.12)下降至(0.22±0.01),且呈剂量效应关系(F=11.73,P=0.000);2)同型半胱氨酸在0.5mmol·L^(-1)开始、干预24h后对人脐静脉内皮细胞增殖具有抑制作用(P<0.05);3)不同浓度同型半胱氨酸干预后TPRV1蛋白表达及细胞增殖能力低于对照组(P<0.05)。结论同型半胱氨酸对人脐静脉内皮细胞的增殖抑制具有剂量效应关系;随同型半胱氨酸浓度增加,TRPV1蛋白表达水平下降,细胞增殖能力亦降低。Objective To explore the effects of homocysteine on the expression of TRPV1 and proliferation and in endothelial cell. Methods After human umbilical endothelial cells（HUVECs）intervened with different concentrations of homocysteine（Hcy）（0,0.05,0.1,0.2,0.5,1,2,4,8 and 16mmol·L^-1）and at different times（6,12,24,36 and 48h）,the proliferation and activity were detected using CCK-8 assay. Then HUVECs were intervened with proper concentrations of homocysteine（0,0.1,0.5,1 and 2mmol·L^-1）based on the previous experiments and the expression of TRPV1 were assessed by Western blot. Results 1）The proliferation of HUVECs were significantly inhibited by homocysteine in a dose-dependent manner and the OD value was declined from（1.03±0.12）to（0.22±0.01）（F=11.73,P=0.000）;2）Homocysteine intervention at 0.5mmol·L^-1 for 24 hours had inhibiting effect on proliferation of human umbilical vein endothelial cells（P〈0.05）;3）The expression of TRPV1 and cell proliferation were significantly different from that of control group（P〈0.05）.Conclusion Homocysteine inhibits the proliferation of human umbilical vein endothelial cells in a dose-dependent mannnerand accompanied with downregulated of TRPV1.

关 键 词：同型半胱氨酸 人脐静脉内皮细胞 增殖 TRPV1蛋白 

分 类 号：R54[医药卫生—心血管疾病]