淫羊藿药渣总糖提取工艺、剩余黄酮含量测定与抗氧化活性研究  

Study on the Optimization of Extraction Technique of Total Sugar and Content Test and Antioxidant Activity of Flavonoids from Herba Epimedii Residue

作　　者：潘雄[1] 李齐激[2] 杨勇[1,2] 杨娟[2] 杨小生 PAN Xiong Li Qiji YANG Yong YANG Juan YANG Xiaosheng(Pharmnceutieal College, Guizhou University, Guiyang 550025, Guizhou, China The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, Guizhou, China)

机构地区：[1]贵州大学药学院 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550014

出　　处：《贵州医科大学学报》2017年第7期767-771,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金项目(81460597);贵州省科技厅农业攻关重点项目[黔科合NY(2015)3005-1号]

摘　　要：目的:了解淫羊藿药渣中总糖与黄酮的残留情况,为淫羊藿药渣的二次开发提供运用依据。方法:以水为提取剂,考察提取温度、提取时间和料液比对淫羊藿药渣总糖提取率的影响;在单因素试验获得最优淫羊藿药渣总糖提取工艺的基础上,采用正交试验确定淫羊藿药渣中总糖的最佳提取工艺,采用紫外-可见分光光度法,以芦丁为对照品,在270 nm处测定吸光度,计算总黄酮的含量并测定其抗氧化活性。结果:提取时间为3 h时淫羊藿药渣总糖提取率最大,2 h略小于3 h,当提取时间再增加时提取率反而有所下降,从时间成本等因素考虑,选择2 h为最佳提取时间;提取温度为90℃时淫羊藿药渣总糖提取率最大,当提取温度再增加,提取率下降,以90℃为最佳提取温度;料液比为1∶30时,淫羊藿药渣总糖提取率最大,以此料液比作为最佳提取条件;影响淫羊藿药渣总糖提取率的各因素主次顺序为提取温度(A)>料液比(C)>提取时间(B),提取温度为100℃,提取时间为3 h,料液比为1∶30时,淫羊藿药渣总糖的提取率为4.03%;淫羊藿药渣中36%左右的黄酮类物质对DPPH自由基有较好的清除作用。结论:选择最佳提取工艺可增加淫羊藿药渣中总糖的提取率,且淫羊藿药渣中一定量的黄酮类物质对DPPH自由基有较好的清除作用。Objective： To investigate the condition of total sugar and flavonoids in the residue of Herba Epimedii and to provide reference for Herba Epimedii residue secondary development. Method： Taking water as extracting agent, investigating effect of extracting temperature, extracting time and feed fluid on extracting ratio of Herba Epimedii residue total sugar. Optimized Herba Epirnedii residue total sugar extracting technique was obtained from single factor test, adopting orthogonal test to determine the best extracting technique of Herba Epimedii residue total sugar; the absorbance was measured at 270 nm using UV-Vis spectrophotometry, rutin as the reference substance. The total tlavonoid content was calculated and antioxidant activity was tested. Result： Best extraction rate of total sugar was 3 h, 2 h condition was slightly less than 3 h condition ; while the increase of extraction time might lead the extraction rate to decrease. Considering time cost, 2 h was the best extraction time. While the extrac- tion temperature was 90 ~C, residue total sugar extraction rate was the best, while the increase of ex- traction temperature might lead the extraction rate would decrease ; 90 ℃ should be the best extraction temperature. When the extracting agent ratio was 1：30, residue total sugar extraction rate was the best; such extracting agent ratio should serve as the best extraction condition; influencing order of resi- due total sugar extraction rate were as follows ： extraction temperature （A） 〉 extraction rate （ C ） 〉 extraction time （B）. At 100℃, extraction time as 3 h, ratio of solid to liquid 1： 30, the extracting rate of total sugar reached 4.03 %. The remaining flavonoids in Herba Epimedii residue reached 36 % and had a better ability for the DPPH free radical scavenging. Conclusion： Optimal extraction technique could increase the extraction rate of residue total sugar extraction rate, while remaining flawmoids in Herba Epimedii residue had a better ability for the DPPH free radical

关 键 词：中草药提取物 淫羊藿药渣 总糖 提取工艺 正交试验 黄酮 抗氧化 

分 类 号：R931.71[医药卫生—生药学] R931.4[医药卫生—药学]