草甘膦对草地早熟禾愈伤组织生长及生理指标的影响  

Effect of glyphosate on growth and physiological index of kentucky bluegrass callus

作　　者：李慧萍[1] 徐海鹏[1] 金宁[2] 金小煜 牛奎举 马晖玲[1] LI Hui-ping XU Hai-peng JIN Ning JIN Xiao-yuv NIU Kui-ju MA Hui-ling(College of Pratacultural Science, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem, Ministry of Education/Pratacultural Engineering Laboratory of Gansu Province /Sino-U. S. Centers for Grazingland Ecosystem Sustainability ,Lanzhou 730070,China Horticultural College, Gansu Agricultural University ,Lanzhou 730070, China)

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃农业大学园艺学院,甘肃兰州730070

出　　处：《草原与草坪》2017年第3期42-47,共6页Grassland and Turf

基　　金：甘肃农业大学大学生科研训练计划(SRTP)项目(20150225);甘肃农业大学国家级大学生创新创业训练计划项目(201510733001);国家自然科学基金"草地早熟禾种间体细胞杂交的研究(31160482)"资助

摘　　要：以草地早熟禾午夜Ⅱ号愈伤组织为试验材料,采用间接接种的方法在草甘膦浓度为0、0.02、0.04、0.06、0.08和0.10mmol/L的MS培养基上继代培养。20d后对愈伤组织进行存活率、相对生长率、褐化情况、电导率、丙二醛、可溶性蛋白、游离脯氨酸含量和抗氧化酶(SOD,POD和CAT)活性的测定。结果表明:愈伤组织的存活率随草甘膦浓度的增大而逐渐下降,相对生长率呈先上升后下降的趋势。电导率、游离脯氨酸含量及SOD,POD和CAT活性呈先上升后下降的趋势,临界点处的草甘膦浓度为0.04mmol/L。丙二醛和可溶性蛋白的临界点草甘膦浓度是0.06mmol/L。说明草地早熟禾午夜Ⅱ号愈伤组织可耐受较小浓度的草甘膦(<0.04mmol/L)。The callus of kentucky bluegrasses（midnightⅡ）was cultured on MS medium which contained different concentrations of glyphosate（0,0.02,0.04,0.06,0.08 and 0.10 mmol/L）.After 20 days,the callus was exploited for measuring physiological indexes（survival rate,relative growth rate,brown case,electrical conductivity,contents of MDA,soluble protein,free proline,SOD,POD and CAT）.The result showed that the survival rate of the callus gradually decreased with the increase of glyphosate,but the relative growth rate increased first and then decreased.0.04 mmol/L of glyphosate concentration was the turning point.While MDA and soluble protein of critical concentrations of glyphosate was 0.06 mmol/L.The tolerable concentration of glyphosate was 0.04 mmol/L.

关 键 词：草甘膦 草地早熟禾 愈伤组织 生理指标 

分 类 号：S688.4[农业科学—观赏园艺]