基于石墨烯基复合物和聚天青Ⅰ的HRP传感器检测有机过氧化物的研究  

Study on Determination of Organic Peroxides with HRP Sensor Based on Graphene Nanocomposites and Poly Azure Ⅰ

作　　者：丁绍卿[1] 杨绍明[1] 李玲玲[1] 杨杰[1] 曹嫱 查文玲[1] 

机构地区：[1]华东交通大学材料学院,江西南昌330013

出　　处：《分析测试学报》2017年第7期865-869,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21465012);江西省自然科学基金项目(20171BAB203017);江西省教育厅科技项目(GJJ150544);华东交通大学校立项目(15LX03)

摘　　要：利用电化学还原氧化石墨烯(GO)的方法将石墨烯(rGO)固定在电极表面上,然后电沉积氢氧化铜和氢氧化镍复合物,构成石墨烯/金属氢氧化物复合纳米材料修饰的玻碳电极(GCE),并通过电聚合天青Ⅰ将辣根过氧化酶(HRP)固定在GCE/rGO/Cu(OH)_2-Ni(OH)_2表面,制得GCE/rGO/Cu(OH)_2-Ni(OH)_2/HRP-PA。对石墨烯/金属氢氧化物复合纳米材料进行了SEM和能谱表征。通过电化学阻抗法和循环伏安法对传感器的制备过程和电化学性能进行了研究,并进一步分别对过氧化氢叔丁基(BHP)及过氧化氢异丙苯(CHP)进行了分析测定。该传感器对BHP和CHP具有良好的检测效果,在2.0×10^(-5)~9.2×10^(-4)mol/L范围内响应电流与BHP浓度呈良好的线性关系,检出限为9.9×10^(-6)mol/L;在3.0×10^(-6)~1.0×10^(-4)mol/L范围内响应电流与CHP浓度呈良好的线性关系,检出限为6.9×10^(-7)mol/L。In this paper,graphene（rGO） was immobilized on the surface of the electrode by the method of electrochemical reduction of graphene oxide（GO）,and then the copper hydroxide-nickel hydroxide nanocomposites were electrodeposited to form graphene/metal hydroxide nanocomposites modified glassy carbon electrode（GCE/rGO/Cu（OH）2-Ni（OH）2）.Finally,horseradish peroxidase（HRP） was immobilized onto the surface of the above prepared electrode by electropolymerizing azure I to form the GCE/rGO/Cu（OH）2-Ni（OH）2/HRP-PA（PA： poly azure Ⅰ）.The graphene/metal hydroxide nanocomposites were characterized by SEM and EDS.The preparation process and electrochemical performance of the sensor were investigated by electrochemical impedance spectroscopy and cyclic voltammetry.Hydrogen peroxide tert-butyl（BHP） and cumene hydroperoxide（CHP） were determined by the sensor.The linear response of the HRP sensor to BHP was in the concentration range of 2.0×10^-5-9.2×10^-4mol/L with a detective limit of 9.9×10^-6mol/L and that to CHP was in the concentration range of 3.0×10^-6-1.0×10^-4mol/L with a detective limit of 6.9×10^-7mol/L.

关 键 词：石墨烯 金属氢氧化物 天青Ⅰ 有机过氧化物 HRP传感器 

分 类 号：O657.1[理学—分析化学] O611.62[理学—化学]