高效液相色谱法测定油梨种子热处理后水溶性糖含量的变化  

Analysis of saccharides in avocado seed after heat treatment by high performance liquid chromatography

作　　者：熊科胜 檀华蓉[1,2] 葛宇[3] 司雄元[1,2] XIONG Ke-sheng TAN Hua-rong GE Yu SI Xiong-yuan(College of Tea ＆ Food Science and Technology, Anhui Agricultural University, Hefei 230036 Biotechnology Center, Anhui Agricultural University, Hefei 230036 Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102)

机构地区：[1]安徽农业大学茶与食品科技学院,合肥230036 [2]安徽农业大学生物科技中心,合肥230036 [3]中国热带农业科学院海口实验站,海口570102

出　　处：《分析试验室》2017年第7期794-798,共5页Chinese Journal of Analysis Laboratory

基　　金：海南省自然科学基金项目(20163096)资助

摘　　要：采用Penomenex氨基柱为分析柱,建立了高效液相色谱法-蒸发光散射检测方法(HPLC-ELSD)测定油梨种子中不同可溶性糖的方法,并比较了热处理对油梨种子中不同可溶性糖的含量的影响。高效液相色谱分析条件的优化条件为乙腈-水(88:12,体积比)为流动相,流速1.0 mL/min,柱温25℃,氮气压力为172 kPa,漂移管温度为60℃。6种糖标准品线性关系良好。检出限为0.09~0.41 mg/L,样品的加标回收率84.5%~104.7%,相对标准偏差(RSD)小于4.9%。油梨种子中的可溶性糖主要由果糖、葡萄糖、D-甘露庚酮糖和甘露庚糖醇组成;油梨种子经热处理后,其中可溶性糖含量均有所下降。A new approach for the determination of different soluble saccharides from avocados seed is described, and effects of heat treatment on the contents of soluble saccharides are investigated. The extracts were analysed by high performance liquid chromatography with evaporative light scattering detector （ELSD）. The soluble saccharides were separated on Penomenex amino column （25 ℃ ） using an acetonitrile-water gradient system at a flow-rate of 1.0 mL/min. The optimum setting of the ELSD parameters： drift tube temperature was 60 ℃ and nitrogen cartier gas pressure was 172 kPa The linearity of six soluble saccharides standards was good. The detection limits were in the range of 0. 09-0. 41 mg/L, and the recoveries ranged from 84. 5% to 104. 7% with deviations （RSD） 〈 4.9%. The soluble saccharides in avocado seed were the relative standard mainly composed of fructose, glucose, D-mannoheptulose and perseitol, and the contents decreased after heat treatment. The method is accurate, reproducible and has been applied for quantitative analysis of soluble saccharides in avocados seed.

关 键 词：高效液相色谱 油梨种子 水溶性糖 热处理 

分 类 号：O657.7[理学—分析化学]