40株院内感染耐甲氧西林金黄色葡萄球菌的耐药性及分子分型研究  

Drug susceptibility and PFGE of 40 Staphylococcus aureus clinical isolates

作　　者：董洪燕[1] 张雪梅[1] 杨建国[1] 马智龙[1] 杨茂丹 

机构地区：[1]泰州市疾病预防控制中心,江苏泰州225300

出　　处：《现代预防医学》2017年第14期2655-2658,2671,共5页Modern Preventive Medicine

基　　金：泰州市科技支撑计划(社会发展)项目(TS201504)

摘　　要：目的了解耐甲氧西林的金黄色葡萄球菌(MRSA)在医院内感染的流行病学特征,为临床MRSA的预防和控制提供依据。方法对从医院内收集的不同来源的MRSA进行生化鉴定、药敏试验及脉冲场凝胶电泳(PFGE)分子分型,并运用Bio Numeries软件分析其亲缘性关系。结果本研究共收集院内感染分离的MRSA 40株。药敏试验表明,40株MRSA对苯唑西林(100.0%)、头孢西丁(100.0%)、青霉素(100.0%)耐药性达最高,其次是红霉素(80.0%),克林霉素(75.0%);多重耐药谱显示3耐以及以上的占100.0%;PFGE分子分型表明40株MRSA共分为36个型,其中3个型别具有100.0%同源性的菌株。结论院内感染分离的MRSA的耐药性强,多重耐药谱广,PFGE带型多样,且有100.0%同源性菌株,表明MRSA在院内存在相互传播的可能。应加强对其耐药性以及溯源分析,为院内感染溯源及预警,减少MRSA的暴发和流行提供实验室的技术支撑。Objective To understand the epidemiological characteristics of MRSA isolates of nosocomial infection, and provide evidence for the control and prevention of MRSA in clinics. Methods MRSA strains were isolated from different sources, identified, and tested for antibiotic sensitivity and typed with PFGE. Taxonomy analysis was conducted by the Bionumerise software. Results 40 MRSA isolates in this study showed resistance of 100% to oxacillin, cefoxitin and penicillin, followed by erythromycin （80%）and clindamycin （75%）. All isolates were resistant to three antibiotics or more. The 40 MRSA isolates were of 36 genotypes by PFGE molecule typing method. Three isolates were completely homologous. Conclusion The MRSA isolates had strong and broad drug resistance. They were diversified in genotypes, but there were still homologous strains, indicating transmission within the hospital. To prevent the outbreak and prevalence of MRSA, we should strengthen the control of drug resistance and source-tracking to reduce nosocomial infections.

关 键 词：院内感染 MRSA 耐药性 PFGE 

分 类 号：R378[医药卫生—病原生物学]