巴马小型猪冠状动脉粥样硬化模型的评价方法  

A reliable system to assess atherosclerosis model of Bama minipigs

作　　者：尹妮[1] 杨关林[1] 姜钧文[2] 王春田[3] 王凤耀[4] 贾连群[5] 高晓宇[1] 潘嘉祥 李芹[1] 李佳[1] 冯元洁 高玉竹[1] 周鹤[1] 张哲[1] YIN Ni YANG Guanlin JIANG Junwen WANG Chuntian WANG Fengyao JIA Lianqun GAO Xiaoyu PAN Jiaxiang LI Qin LI Jia FENG Yuanjie GAO Yuzhu ZHOU He ZHANG Zhe(Blood-vessel Laboratory, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China Department of Cardiology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China Laboratory Animal Center, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China Department of Anesthesia, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China Platform of Scientific Research, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China)

机构地区：[1]辽宁中医药大学附属医院血脉病研究室,辽宁沈阳110032 [2]辽宁中医药大学附属医院心内科,辽宁沈阳110032 [3]辽宁中医药大学实验动物中心,辽宁沈阳110847 [4]辽宁中医药大学附属医院麻醉科,辽宁沈阳110032 [5]辽宁中医药大学重大科研平台,辽宁沈阳110847

出　　处：《山东大学学报（医学版）》2017年第7期1-5,48,共6页Journal of Shandong University:Health Sciences

基　　金：国家重点基础研究发展计划(2013CB531704)

摘　　要：目的从可行性、稳定性和安全性等方面对巴马小型猪冠状动脉粥样硬化模型进行评价。方法巴马小型猪随机分为对照组(给予基础饲料)和模型组(给予高脂喂饲联合机械损伤),在0、24、48周分别对两组行冠状动脉造影、血管内超声(IVUS)、心电图、血脂、炎症指标检测和病理学分析。结果 24周模型组总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、氧化低密度脂蛋白(ox-LDL)、高敏C反应蛋白(hs-CRP)、白介素-6(IL-6)、血管细胞黏附分子-1(VCAM-1)、单核细胞趋化因子-1(MCP-1)和P选择素(s P-selectin)明显高于对照组(P<0.05);模型组0、24周ST段下移程度与对照组相比差异无统计学意义(P>0.05),模型组0周T波振幅与对照组相比差异无统计学意义(P>0.05),模型组24周T波振幅与对照组相比差异有统计学意义(P<0.05);24、48周冠状动脉造影和IVUS显示模型组冠脉平均面积狭窄率高于对照组;48周模型组血管内膜厚度和内膜中膜厚度比大于24周。结论离体在体结合评价是一种可行、安全的评价冠状动脉粥样硬化模型的方法。Objective To evaluate the feasibility, stability and safety of a coronary atherosclerosis model of Bama minipigs. Methods Bama minipigs were randomly assigned into control group （basic diet） and model group （high-fat diet combined with mechanical injury）. In week 0, 24 and 48, both groups underwent coronary angiography, intravas- cular ultrasound （IVUS）, electrocardiogram, serum lipid testing, inflammation factors testing, and pathological analysis to evaluate the model. Results In week 24, compared with the control group, the model group had significantly in- creased total cholesterol （ TC）, triglycerides （ TG）, low density lipoprotein cholesterol （ LDL-C）, high density lipo- protein cholesterol （HDL-C）, oxidized low-density lipoprotein （ox-LDL）, high-sensitivity C-reactive protein （hs- CRP）, interleukin-6 （lL-6）, vascular cell adhesion molecule-1 （VCAM-1）, monocyte chemotactic protein （MCP-1） and soluble P-selectin （SP-selectin） （ P 〈 0.05 ）. There was no statistically significant difference between the two groups in ST segment depression in week 0 and 24 （ P 〉 0.05 ）. There was no statistically significant difference between the two groups in T waves in week 0 （ P 〉 0.05 ）, but there was statistically significant difference in T waves in week 24 （P 〈 0.05 ）. In week 24 and 48, the model group showed significantly higher coronary stenosis rate than the control group. In week 48, the model group had higher intima/intima-medial thickness ratio than in week 24. Conclusion The in vitro and in vivo model is safe and feasible in the assessment of atherosclerosis.

关 键 词：TatPTD-内皮抑素 壳聚糖 纳米基因载体 基因治疗 

分 类 号：R332[医药卫生—人体生理学]