桂枝温通经脉配伍对胶原诱导性关节炎大鼠的免疫调节作用  

Immunomodulatory Effects of Compatibilities of Ramulus Cinnamomi for Warming Meridians on Collageninduced Arthritis Rats

作　　者：彭代平[1] 汤小虎[2,3] 周瑞彬[1] 

机构地区：[1]云南省昆明市中医医院心血管内分泌病科,昆明650011 [2]云南省中医医院风湿免疫病科,昆明650021 [3]云南中医学院临床医学院,昆明650500

出　　处：《中国中西医结合杂志》2017年第7期813-818,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目(No.81060285);云南省应用基础研究项目(No.2010ZC192);云南省卫生科技项目(No.2010NS104);云南省教育厅科学研究基金项目(No.2013J044);云南省高校科技创新团队支持计划资助(No.IRTSTYN)

摘　　要：目的研究桂枝温通经脉配伍对胶原诱导性关节炎(CIA)模型大鼠的治疗作用,并探讨其作用机制。方法雄性Wistar大鼠110只,随机分为正常组10只,其余于大鼠尾根及背部每只0.5 mg皮内注射胶原乳剂制备关节炎大鼠模型,致炎后21天,将造模成功的大鼠(90只)随机分为模型组、甲氨喋呤组、桂枝组、桂枝牛膝组、桂枝川乌组、桂枝独活组、桂枝附子低、中、高剂量组,每组10只;分别灌胃给药,每天1次,连续21天。观察大鼠关节炎指数(AI)、后爪体积及踝关节病理变化情况,并检测血清中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)含量。结果与模型组比较,桂枝牛膝组、桂枝川乌组、桂枝独活组、桂枝附子高、中剂量组及甲氨喋呤组关节炎大鼠AI及后爪肿胀明显降低(P<0.05);桂枝牛膝组、桂枝川乌组、桂枝独活组、桂枝附子中、高剂量组及甲氨喋呤组关节炎大鼠血清TNF-α的含量显著下调(P<0.01),并对踝关节病理变化有改善;各给药组大鼠血清IL-6的含量均显著降低(P<0.01)。结论桂枝温通经脉配伍对CIA大鼠关节炎症有一定的抑制作用,可能是治疗类风湿关节炎的有效配伍。Objective To observe the therapeutic effects of the compatibilities of Ramulus Cinnamomi （RC） for warming meridians on collagen-induced arthritis （CIA） rats, and to study its mechanisms. Methods Totally 110 male Wistar rats were randomly divided into the normal group （n =10） and the CIA modeling group （n = 100）. The CIA model was established by intradermally injecting collagen emulsion （0.5 mg） from tail and back. At day 21 after induction, successfully modeled rats （90 male） were randomly divided into the CIA model group, the methotrexate （MTX） group, the RC group, RC Achyranthes Bidentata group （A）, RCRadix Aconiti group （B）, RCAngelica Pubescens group （C）, and the low, middle, high dose RCAconitum carmichaeli Debx.group （D, E, F）, 10 rats in each group. All rats were intragastrically administered, once per day for 21 successive days. Arthritis index （AI）, paw volume, and pathological changes of ankle joint were observed after treatment. The serum levels of TNF- α, IL-1β, and IL-6 were measured. Results Compared with the CIA model group, AI and the swelling degree of the joints was significantly alleviated in Group A, B, C, E, F, and the MTX group （P 〈0.05）. The serum content of TNF-α was significantly down-regulated in Group A, B, C, E, F, and the MTX group （P 〈0.01 ）. Pathological changes of ankle joint were also improved as well. Serum levels of IL-6 were de

关 键 词：桂枝 温通经脉 炎性细胞因子 类风湿关节炎 

分 类 号：R285.5[医药卫生—中药学]