电针不同穴位对大鼠分娩镇痛效应及其神经递质作用的影响  

Effect of Electroacupuncture at Different Acupoints on Delivery Analgesic Effect and Neurotransmitters in Rats

作　　者：沈岩金[1] 蒋秋燕[1] 莫海霞[1] 李丽[1] 王梦莹[1] 

机构地区：[1]广西中医药大学第一附属医院产科,南宁530023

出　　处：《中国中西医结合杂志》2017年第7期833-839,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81260547;No.81373685)

摘　　要：目的探讨电针不同穴位对大鼠分娩镇痛效应及其神经递质的作用机制。方法 120例模型孕鼠随机分为空白组(A组),电针三阴交穴组(B组),电针合谷穴组(C组),电针合谷加三阴交组(D组),电针血海穴组(E组),药物组(F组),每组20只。采用热水甩尾测痛法观察大鼠痛阈值;Real-time PCR及Western blot检测大鼠中枢神经递质5羟色胺(5-HT)及5羟色胺2A受体(5-HT2A)、去甲肾上腺素转运蛋白(NET)及去甲肾上腺素α2-A受体(α2-AR)、多巴胺(DA)及多巴胺D2受体(Drd2)、前强啡肽原(Pdyn)及阿片受体(Oprk1)的mRNA与蛋白表达。结果 (1)大鼠痛阈值比较,干预前各组间差异无统计学意义(P>0.05);干预后,与A组比较,B、C、D、E、F组痛阈明显升高(P<0.01)。(2)与A组比较,B、C、D、E、F组中枢神经中大脑灰质5-HT、5-HT2A及NET、α2-AR mRNA及蛋白表达均明显升高(P<0.01);DA、Drd2均明显降低(P<0.01);Pdyn、Oprk1差异无统计学意义(P>0.05)。而脊髓中5-HT、5-HT2A及Pdyn、Oprk1明显升高(P<0.01);NET、α2-AR明显降低(P<0.01);DA、Drd2差异无统计学意义(P>0.05)。结论不同穴位产生不同针效作用,以B组较好,E组相对较差。穴位间出现的这种差异,可能与穴位所在的经络腧穴功效及其所处的神经解剖学位置有关。Objective To observe delivery analgesic effect of electroacupuncture （EA） at different acupoints and the mechanism of neurotransmitters in rats. Methods Totally 120 pregnant model rats were randomly divided into a blank control group （A）, EA at Sanyinjiao （SP6） group （B）, EA at Hegu （LI4） group （C）, EA at Hegu （LI4） and Sanyinjiao （SP6） group （D）, EA at Xuehai （SP10） group （E）, and drug group （F） 20 in each group. The pain threshold of rats were observed using hot water tail flick test. Real-time PCR and Western blot were used to detect mRNA and protein expressions of rat central neurotransmitters and their receptors （5-HT and 5-HT2A, NET and （x2-AR, DA and Drd2, Pdyn and Oprk1）. Results （1） There was no significant difference in pain threshold among all the groups before intervention （P〉0.05）. Compared with group A, the pain threshold significantly increased in group B, C, D, E, and F （P 〈0.01 ）. （2） 5-HT, 5-HT2A, NET,α2-AR of gray matter in the central nervous system significantly increased （P 〈0.01） ; DA and Drd2 significantly decreased （P 〈0.01 ） in group B, C, D, E, and F, as compared with group A. But there was no statistical difference in Pdyn and Oprkl between group A and the rest groups （P 〉0.05）. Compared with group A, 5-HT and 5-HT2A, Pdyn, and Oprk1 in the spinal cord increased significantly （P 〈0.01 ） ; NET and α-AR significantly decreased （P 〈0.01 ） in the rest groups. There was no statistical difference in DA and Drd2 between group A and the rest groups （P 〉0.05）. Conclusions Different acupuncture points had different acupuncture effects. Better results were obtained in group B, while poorer results were obtained in group E. This difference between the acu- points might be related to the actions of meridian acupoints and the neuroanatomical sites.

关 键 词：电针穴位 分娩镇痛 神经递质及其受体 

分 类 号：R245[医药卫生—针灸推拿学]