不同频率电针对神经病理性疼痛诱发焦虑大鼠ACC区磷酸化ERK水平的影响  

Effects of Electroacupuncture with Different Frequencies on p-ERK Levels in ACC of Neuropathic Pain Induced Anxiety Rats

作　　者：寿升芸[1,2] 邵晓梅[1] 沈醉 林小溪[1] 叶佳瑜 魏骏骏 庄晟坚 蒋永亮[1] 方剑乔[1] 

机构地区：[1]浙江中医药大学第三临床医学院针灸神经生物学实验室,杭州310053 [2]浙江省杭州市余杭区第二人民医院中医科,杭州311121

出　　处：《中国中西医结合杂志》2017年第7期840-846,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81574056);浙江省自然科学基金资助项目(No.LY15H270009);浙江省新苗人才计划项目(No.2014R410056);浙江省医药卫生科技项目省部共建项目(No.WKJ-ZJ-1419);方剑乔全国名老中医专家传承工作室(No.GZS2012014;国中医药人教发[2013]47号)

摘　　要：目的观察不同频率电针对神经病理性痛诱发焦虑大鼠前扣带回皮层(anterior cingulate cortex,ACC)细胞外信号调节激酶(extracellular signal-regulated kinase,ERK)磷酸化(p-ERK)水平的影响,探讨电针治疗神经病理痛诱发焦虑的最佳频率及其可能机制。方法将48只SD大鼠随机分为空白对照组(8只)、假手术组(8只)、模型组(8只)、2 Hz电针组(8只)、15 Hz电针组(8只)和100 Hz电针组(8只)。将其中32只大鼠建立L5脊神经结扎(spinal nerve ligation,SNL)模型,假手术组仅分离L5脊神经,但不进行结扎。2、15、100 Hz电针组在造模后选用双侧"环跳"、"阳陵泉"穴,并分别用2、15、100 Hz进行电针治疗,隔日1次,每次30 min,共13次。采用动态足底触觉仪检测大鼠双侧后足缩足阈(paw withdrawal thresholds,PWTs)作为机械痛阈;高架O型迷宫试验(elevated zero maze test,EZMT)评估大鼠的焦虑水平;Western blot法测ACC区的p-ERK水平。结果 PWTs:SNL术后第3天,模型组大鼠的患侧PWTs均明显低于假手术组(均P<0.01);2、15、100 Hz电针组大鼠术后第8、12、16、20、28天的患侧PWTs呈持续升高趋势,均高于同时点的模型组(P<0.01);且2 Hz电针组大鼠患侧PWTs较15 Hz电针组和100 Hz电针组高(P<0.01)。EZMT评分:与假手术组比较,模型组大鼠开放臂活动时间(open arm time,OAT)明显减少(P<0.05);15、100 Hz电针组OAT明显多于模型组(P<0.05);而2 Hz电针组OAT与模型组比较,差异无统计学意义(P>0.05)。ACC内p-ERK蛋白表达:模型组患侧ACC区p-ERK蛋白表达水平明显高于假手术组(P<0.01),而2、15、100 Hz电针组患侧ACC区p-ERK蛋白表达水平明显低于模型组(P<0.01)。且100 Hz电针组患侧ACC区p-ERK蛋白表达水平明显低于2、15 Hz电针组(P<0.01,P<0.05)。结论电针干预神经病理痛和其诱发的焦虑情绪的最佳频率不同。2 Hz电针治疗神经病理痛效果更好,但对于神经病理痛诱发的焦虑情绪,100 Hz疗效更优。其中,100 Hz电针对�Objective To observe the effects of electroacupuncture （EA） with different frequencies on the expression of phosphorylated extracellular signal-regulated kinase （p-ERK） in anterior cingulate cortex （ACC） of rats in neuropathic pain induced anxiety rats, and to study the optimal frequency of EA for neuropathic pain induced anxiety and its possible mechanism. Methods Totally 48 SD rats were randomly divided into 6 groups, i.e., the normal group, the sham-operation group, the model group, 2 Hz EA group, 15 Hz EA group, and 100 Hz EA group, 8 in each group. Spinal nerve ligation （SNL） model was established in 32 rats （L5）. Of them, lumbar 5 spinal nerve was separated in the sham-operation group, but without ligation. Rats in 2 Hz, 15 Hz, and 100 Hz EA group were respectively needled at bilateral Huantiao （GB30） and Yanglingquan （GB34） with 2 Hz, 15 Hz, and 100 Hz, once every other day, 30 min each time for a total of 13 times. Paw withdrawal thresholds （PWT） was detected as mechanical pain threshold using Dynamic Plantar Aesthesiometer. Elevated zero maze test （EZMT） was used to evaluate the level of anxiety of rats. And then Western Blot was used to determine the expression of p-ERK in ACC. Results The PWTs showed, as compared with the sham-operation group, the ipsilateral PWTs were obviously shorter in the model group than in the sham-operation group at day 3 after SNL （P 〈 0.01 ）. The ipsilateral PWTs showed an increasing tendency in rats of 2 Hz, 15 Hz, and 100 Hz EA groups at day 8, 12, 16, 20, and 28 after operation, higher than those of the model group at the same time points （all P 〈0.01 ）. The ipsilateral PWTs in rats of 2 Hz EA group were higher than those of 15 Hz EA group and 100 Hz EA group at the same time points （alIP 〈0.01 ）. Scores in EZMT showed, as compared with the sham-operation group, open arm time （OAT） was reduced more obviously in rats of the model group （P 〈0.05）. Besides, OAT in rats of 15 Hz EA group and 100 Hz EA group were o

关 键 词：电针 神经病理痛 焦虑 磷酸化细胞外信号调节激酶 

分 类 号：R245[医药卫生—针灸推拿学]