生发片通过激活PKA信号通路促进小鼠B16黑素瘤细胞黑素合成实验研究  

Research on the Effect of Sheng Fa Tablets in Promoting the Melanogenesis in B16 Melanoma Cells through PKA Pathway

作　　者：曲珍仪 李明[1] 胡燕 贾世伟 黄少鹏[1] 刘颖[1] Qu Zhenyi Li Ming Hu Yan Jia Shiwei Huang ShaopengI Liu Ying(College of Traditional Chinese Medicine of Guang Dong Pharmaceutical University ,Guangzhou 510006,China Bai Hui Pharmaceutical Co. Ltd. ,Nanning 530000,China)

机构地区：[1]广东药科大学,广东广州510006 [2]南宁百会药业有限公司,广西南宁530000

出　　处：《亚太传统医药》2017年第14期3-5,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:探讨生发片组方水提物对小鼠B16黑素瘤细胞酪氨酸酶活性和黑素合成的影响及作用机制。方法:采用MTT法筛选合适的药物浓度,L-多巴氧化法检测酪氨酸酶活性,氢氧化钠裂解法检测黑素生成,Western blotting检测MITF、TYR及p-CREB蛋白的表达水平。结果:与对照组相比,在0.25~1g·L-1浓度范围内,生发片组方水提物可明显促进酪氨酸酶活性和黑素合成,上调MITF、TYR及p-CREB蛋白的表达。结论:生发片水提物可能通过PKA信号通路促进黑素的合成。Objective：To research the effect and mechanism of sheng fa tablet aqueous extract on tyrosinase activity and melanogen- esis in B16 melanoma cells. Methods： The appropriate concentration screened with MTT; The tyrosinase activity was detected by L- DOPA-Oxidation;the amount of melanin was detected by NaOH dissolvedmethod;the expression of MITF, TYR and p-CREB was detected by Western blotting. Results：Range 0.25g·L-1 to 1g·L-1 ,the tyrosinase activity,the amount of melanin and the expression of MITF,TYR and p-CREB in sheng fa tablet group were higher than those in control group. Conclusion：Sheng fa tablet can promot the melanogenesis in B16 melanoma cells through the PKA pathway.

关 键 词：生发片 黑素 作用机制 

分 类 号：R285[医药卫生—中药学]