电针延缓HepG2裸鼠荷瘤生长趋势及对PCNA表达影响  

Electroacupuncture Delaying Tumor Growth and its Effect on PCNA Expression in HepG2 Nude Mice

作　　者：王睿[1] 余陈欢[2] 方杰[2] 曹畅[1] 

机构地区：[1]杭州市中医院,杭州310007 [2]浙江省医学科学院,杭州310007

出　　处：《上海针灸杂志》2017年第7期852-856,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：浙江省中医药科技计划项目(2015ZA161);浙江省院所专项(2015D3006)

摘　　要：目的观察电针干预对Hep G2裸鼠荷瘤生长趋势及PCNA表达的影响。方法将32只裸鼠随机分为电针组、电刺激组、模型组与空白组,每组8只。采用HepG2建立皮下荷瘤裸鼠模型。电针组采用双下肢足三里+三阴交穴进行电针干预,电刺激组取相同穴位进行直接电流刺激。观测荷瘤体积变化、瘤重比和肿瘤生长抑制率,并检测荷瘤组织PCNA的表达水平。结果电针组、电刺激组和模型组干预前后荷瘤体积比较,差异均无统计学意义(P>0.05);电针组、电刺激组干预后荷瘤体积与模型组比较,差异均无统计学意义(P>0.05),但电针组荷瘤体积生长趋势较缓。各组干预前后裸鼠体重比较,差异均无统计学意义(P>0.05)。电针组干预后瘤重比与电刺激组和模型组比较,差异均有统计学意义(P<0.05);但电刺激组干预后瘤重比与模型组比较,差异无统计学意义(P>0.05)。电针组干预后肿瘤生长抑制率与电刺激组比较,差异有统计学意义(P<0.05)。电针组和电刺激组干预后PCNA表达水平与模型组比较,差异均有统计学意义(P<0.05);但电针组干预后PCNA表达水平与电刺激组比较,差异无统计学意义(P>0.05)。结论电针干预在一定程度上可延缓Hep G2荷瘤生长趋势。Objective To explore the effect of electroacupuncture intervention on tumor growth and PCNA expression in HepG2 nude mice.Methods Thirty-two nude mice were randomized to electroacupuncture, electrostimulation, model and blank control groups, 8 mice each. A nude mouse model of subcutaneous tumor was made with HepG2. The electroacupuncture group received electroacupuncture at points Zusanli and Sanyinjiao on bilateral lower limbs and the electrostimulation group, direct electrical stimulation of the same points. The tumor volume, the tumor/weight ratio and the tumor growth inhibition rate were observed. PCNA expression in the tumor tissue was determined.Results There was no statistically significant pre-/post-intervention difference in the tumor volume in the electroacupuncture, electrostimulation and model groups （P〉0.05）. There was no statistically significant post-intervention difference in the tumor volume between the electroacupuncture or electrostimulation group and the model group （P〉0.05）, but tumor growth tended to slow in the electroacupuncture group. There was no statistically significant pre-/post-intervention difference in the nude mouse weight in every group （P〉0.05）. There was a statistically significant post-intervention difference in the tumor/weight ratio between the electroacupuncture group and the electrostimulation or model group （P〈0.05）, but no statistically significant post-intervention difference in the tumor/weight ratio between the electrostimulation and model groups （P〉0.05）. There was a statistically significantpost-intervention difference in the tumor growth inhibition rate between the electroacupuncture and electrostimulation groups （P＆lt;0.05）. There was a statistically significant post-intervention difference in the PCNA expression level between the electroacupuncture or electrostimulation group and the model group （P＆lt;0.05）, no statistically significant post-intervention difference in the PCNA expression level between the electroacupunctu

关 键 词：电针 电刺激 HepG2荷瘤 PCNA 裸鼠 

分 类 号：R203[医药卫生—中医学]