猪流行性腹泻病毒山西分离株S基因遗传变异分析  

Genetic Variation Analysis of S Gene of Porcine Epidemic Diarrhea Virus Isolated in Shanxi Provine

作　　者：刘文俊[1] 姚敬明[1] 吴忻[1] 孟帆[1] 韩一超[1] 王娟萍[1] 樊振华[1] 薛翼鹏[1] 米瑞娟[1] 李红丽[1] 赵岳[1] 

机构地区：[1]山西省农业科学院畜牧兽医研究所,太原030032

出　　处：《中国畜牧兽医》2017年第7期1982-1989,共8页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技攻关项目(猪流行性腹泻分子流行病学调研与防控技术研究20140311020-2-A);山西省科技攻关项目(山西猪流行性腹泻病原研究及防控对策20150311018-3)

摘　　要：为分析山西地区猪流行性腹泻病毒(PEDV)的遗传变异情况,试验利用RT-PCR方法对2014-2015年山西省疑似猪流行性腹泻的阳性病料进行克隆和测序,获得4个S基因片段,并对其基因序列和推导的氨基酸序列与国内外毒株进行比对分析。序列分析结果显示,4株PEDV山西分离株的S基因与CV777vaccine相比,在170~171bp之间插入12个核苷酸,在401~402、454~455bp之间均插入3个核苷酸,在461~468bp之间缺失6个核苷酸。4株PEDV山西分离株S基因之间核苷酸和氨基酸同源性分别为99.2%~99.8%和98.6%~99.7%,与2011-2015年中国流行毒株、CV777vaccine、attenuated DR13、CV777的核苷酸同源性分别95.0%~98.5%、93.2%~93.6%、93.2%~93.7%、93.7%~94.4%,氨基酸同源性分别为96.2%~98.9%、91.9%~92.6%、92.1%~92.9%、92.9%~94.0%。遗传进化树分析结果表明,PEDVS基因分为3个群,4株PEDV山西分离株属于第一群,与2010年以后国内流行毒株(除AH-M、SQ2014)的亲缘关系较近,与2010年以前中国流行毒株、2个日本株、7个韩国株、2个疫苗株的亲缘关系较远。研究结果提示山西省流行的PEDV发生较明显的变异,需研发新的疫苗来控制PEDV的暴发。In order to investigate the variation in S gene of porcine epidemic diarrhea virus（PEDV）,the 4strains of PEDV Sgene nucleotide sequences were obtained,through RT-PCR amplification of tissue samples from Shanxi province.The obtained sequences and the deduced amino acid were analyzed and compared with the other published PEDV strains.Sequence analysis showed that compared with CV777 vaccine,there were 12 nucleotides insertions between 170 to 171bp,3nucleotides insertions between 401 to 402and 454 to 455bp,6nucleotides deletion between 461 to 468bp.The nucleotide and amino acid homologies were 99.2% to 99.8% and98.6%to 99.7%respectively among 4strains of PEDVSgene;Comparing with the strains isolated from China in 2011 to 2015,CV777 vaccine,attenuated DR13 and CV777,the nucleotide homologies were 95.0%to 98.5%,93.2% to 93.6%,92.1% to 92.9%,93.7% to 94.4%,respectively.The amino acid homology were 96.2%to 98.9%,91.9%to 92.9%,91.9%to 92.6%,92.9%to 94.0%,respectively.Phylogenetic analysis revealed that 4strains of PEDVSgene belonged to the first group and had high correlative genetic relationship with the PEDV strains which isolated after 2010 in China,and had far correlative genetic relationship with the PEDV strains which isolated before 2010 in China,2strains of Japanese,7strains of South Korea,2vaccine strains.The results suggested that the prevalence of PEDV in Shanxi province had a more obvious variation.Therefore,it was necessary to develop a new vaccine to control the outbreak of PEDV.

关 键 词：猪流行性腹泻病毒 S基因 序列分析 

分 类 号：S858.28[农业科学—临床兽医学]